H2-Dk Antibody (15-5-5): sc-52543
- H2-Dk Antibody (15-5-5) is a mouse monoclonal IgG2a H2-Dk antibody provided at 100 µg/ml
- raised against C3H splenocytes of mouse origin
- recommended for detection of H2-Dk class I alloantigen of mouse origin by FCM; may cross-react with H2-Kd and with cells from mice with the H2f haplotype; non cross-reactive with other haplotypes
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for H2-Dk Antibody (15-5-5). This work is in progress.
H2-Dk Antibody (15-5-5) is a mouse monoclonal IgG2a antibody that detects H2-Dk protein of mouse origin by flow cytometry (FCM). Anti-H2-Dk antibody (15-5-5) is available in both non-conjugated and various conjugated forms, including fluorescein isothiocyanate (FITC), providing flexibility for different experimental needs. H2-Dk protein is a crucial component of the major histocompatibility complex (MHC) class I molecules, which play a vital role in the immune response by presenting endogenous peptides to cytotoxic T lymphocytes (CTLs). Located on the surface of nearly all nucleated cells, H2-Dk protein is essential for the recognition of infected or malignant cells by the immune system, thereby facilitating targeted immune responses. The structure of H2-Dk, which consists of a heavy chain and a light chain (β2-microglobulin), allows for effective binding and presentation of a diverse array of peptide antigens, influencing T cell activation and differentiation. Understanding the structural nuances of H2-Dk protein is important for developing immunotherapies and vaccines, as these features can impact the efficacy of T cell-mediated responses against various diseases.
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H2-Dk Antibody (15-5-5) References:
- Effects of altered antigen processing on H-2Dk mediated NK inhibition in a murine T lymphoma model. | Van Ginderachter, J., et al. 1998. Adv Exp Med Biol. 451: 237-40. PMID: 10026878
- Differential expression of the H-2Dk MHC class-I antigen and tissue inhibitor of metalloproteinases in metastatic and nonmetastatic T-10 fibrosarcoma cells. | Har-Vardi, I., et al. 1992. Invasion Metastasis. 12: 301-8. PMID: 1298741
- A molecular model of MHC class-I-restricted antigen processing. | Monaco, JJ. 1992. Immunol Today. 13: 173-9. PMID: 1386516
- Identification of the immunodominant HY H2-D(k) epitope and evaluation of the role of direct and indirect antigen presentation in HY responses. | Millrain, M., et al. 2005. J Immunol. 175: 7209-17. PMID: 16301625
- H2Kk can influence whether cytotoxic T lymphocytes recognize trinitrophenyl in association with H2Dk unique or H-2Kk and H-2Dk shared self determinants. | Fujiwara, H., et al. 1982. J Immunol. 129: 1189-93. PMID: 6179996
- Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. | Ozato, K., et al. 1980. J Immunol. 124: 533-40. PMID: 7188699
- Peptides naturally presented by MHC class I molecules. | Rammensee, HG., et al. 1993. Annu Rev Immunol. 11: 213-44. PMID: 8476560
- Resistance to polyoma virus-induced tumors correlates with CTL recognition of an immunodominant H-2Dk-restricted epitope in the middle T protein. | Lukacher, AE. and Wilson, CS. 1998. J Immunol. 160: 1724-34. PMID: 9469430
- The nature of the MHC class I peptide loading complex. | Cresswell, P., et al. 1999. Immunol Rev. 172: 21-8. PMID: 10631934
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
H2-Dk Antibody (15-5-5) | sc-52543 | 100 µg/ml | $322.00 | |||
H2-Dk Antibody (15-5-5) FITC | sc-52543 FITC | 100 tests in 2 ml | $336.00 |