Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
h-prune Antibody (F-5): sc-393318. Western blot analysis of h-prune expression in WiDr (A) and A-673 (B) whole cell lysates. Detection reagent used: m-IgGκ BP-HRP: sc-516102.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
h-prune Double Nickase Plasmid (h): sc-412914-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- h-prune Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- h-prune Double Nickase Plasmid (h) and h-prune Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRUNE1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: h-prune Antibody (F-5): sc-393318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
h-prune Double Nickase Plasmid (h) | sc-412914-NIC | 20 µg | $410.00 | |||
h-prune Double Nickase Plasmid (h2) | sc-412914-NIC-2 | 20 µg | $410.00 |