H⁺/K⁺ ATPase β Antibody (B-5): sc-376393

H+/K+ ATPase β Antibody (B-5) is a mouse monoclonal IgG2a antibody that detects H+/K+ ATPase β in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). H+/K+ ATPase β, also known as ATP4B or ATP6B, is a crucial 291 amino acid single-pass type II membrane protein that serves as the β subunit of the H+/K+ ATPase heterodimer, working in concert with the α subunit to facilitate ATP hydrolysis while exchanging hydrogen and potassium ions across the plasma membrane. This process is vital for gastric acid secretion, which is essential for digestion and maintaining the acidic environment of the stomach. H+/K+ ATPase β location in the plasma membrane enables direct transport of ions, thereby influencing gastric pH and overall digestive health. H+/K+ ATPase β gene is located on human chromosome 13, a region containing over 400 genes, including critical tumor suppressor genes like BRCA2 and RB1, highlighting H+/K+ ATPase β significance not only in gastric physiology but also in broader biological contexts.

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H⁺/K⁺ ATPase β Antibody (B-5) References:

1. Identification of a gastritogenic epitope of the H/K ATPase beta-subunit.  |  De Silva, HD., et al. 1999. Immunology. 96: 145-51. PMID: 10233689
2. The roles of carbohydrate chains of the beta-subunit on the functional expression of gastric H(+),K(+)-ATPase.  |  Asano, S., et al. 2000. J Biol Chem. 275: 8324-30. PMID: 10722662
3. The K(+) affinity of gastric H(+),K(+)-ATPase is affected by both lipid composition and the beta-subunit.  |  Hermsen, HP., et al. 2000. Biochim Biophys Acta. 1480: 182-90. PMID: 11004562
4. Defining T cell receptors which recognise the immunodominant epitope of the gastric autoantigen, the H/K ATPase beta-subunit.  |  De Silva, HD., et al. 2000. Autoimmunity. 33: 1-14. PMID: 11204248
5. Mutational study on the roles of disulfide bonds in the beta-subunit of gastric H+,K+-ATPase.  |  Kimura, T., et al. 2002. J Biol Chem. 277: 20671-7. PMID: 11909858
6. Plasma membrane delivery of the gastric H,K-ATPase: the role of beta-subunit glycosylation.  |  Vagin, O., et al. 2003. Am J Physiol Cell Physiol. 285: C968-76. PMID: 12773316
7. The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit.  |  Duffield, A., et al. 2003. Proc Natl Acad Sci U S A. 100: 15560-5. PMID: 14660791
8. Contribution of oligosaccharides to protection of the H,K-ATPase beta-subunit against trypsinolysis.  |  Crothers, JM., et al. 2004. Electrophoresis. 25: 2586-92. PMID: 15300779
9. E2P state stabilization by the N-terminal tail of the H,K-ATPase beta-subunit is critical for efficient proton pumping under in vivo conditions.  |  Dürr, KL., et al. 2009. J Biol Chem. 284: 20147-54. PMID: 19491099
10. Positive regulation of the enzymatic activity of gastric H(+),K(+)-ATPase by sialylation of its β-subunit.  |  Fujii, T., et al. 2016. Biochim Biophys Acta. 1858: 1228-35. PMID: 26922883

The preferred H⁺/K⁺ ATPase β antibody is H⁺/K⁺ ATPase β Antibody (C-4): sc-374094.