Date published: 2025-9-27

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H-L-Pro-pNA Trifluoracetate (CAS 108321-19-3)

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Alternate Names:
L-Proline p-nitroanilide trifluoroacetate salt; P-pNA; Pro-pNA
CAS Number:
108321-19-3
Molecular Weight:
349.26
Molecular Formula:
C11H13N3O3•C2HF3O2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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H-L-Pro-pNA Trifluoracetate is a chemical utilized in biochemical research, primarily as a chromogenic substrate for the detection and quantitative analysis of protease enzyme activity. Specifically, it is designed to be a substrate for prolyl endopeptidase, an enzyme that cleaves peptide bonds at proline residues. Upon cleavage by the target protease, H-L-Pro-pNA Trifluoracetate releases p-nitroaniline, a compound that exhibits a yellow color and can be quantified spectrophotometrically. This property allows researchers to monitor enzyme kinetics and to measure the activity of proteases in various biological samples. Moreover, it is instrumental in the screening of enzyme inhibitors, as changes in the rate of p-nitroaniline release reflect the inhibitory effects on the enzyme being studied.


H-L-Pro-pNA Trifluoracetate (CAS 108321-19-3) References

  1. Screen-printed amperometric microcell for proline iminopeptidase enzyme activity assay.  |  Nagy, G., et al. 2000. Biosens Bioelectron. 15: 265-72. PMID: 11219738
  2. Characterization of a novel cell wall-anchored protein with carboxylesterase activity required for virulence in Mycobacterium tuberculosis.  |  Lun, S. and Bishai, WR. 2007. J Biol Chem. 282: 18348-18356. PMID: 17428787
  3. Defining the bacteroides ribosomal binding site.  |  Wegmann, U., et al. 2013. Appl Environ Microbiol. 79: 1980-9. PMID: 23335775
  4. Optimization of poly(N-isopropylacrylamide) as an artificial amidase.  |  Wong, YM., et al. 2015. Biomacromolecules. 16: 411-21. PMID: 25488109
  5. Enzyme-Mimic Peptide Assembly To Achieve Amidolytic Activity.  |  Wong, YM., et al. 2016. Biomacromolecules. 17: 3375-3385. PMID: 27642764
  6. A dual role for proline iminopeptidase in the regulation of bacterial motility and host immunity.  |  Kan, J., et al. 2018. Mol Plant Pathol. 19: 2011-24. PMID: 29517846

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

H-L-Pro-pNA Trifluoracetate, 100 mg

sc-285986
100 mg
$100.00

H-L-Pro-pNA Trifluoracetate, 1 g

sc-285986A
1 g
$129.00