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H-L-Pro-pNA Trifluoracetate is a chemical utilized in biochemical research, primarily as a chromogenic substrate for the detection and quantitative analysis of protease enzyme activity. Specifically, it is designed to be a substrate for prolyl endopeptidase, an enzyme that cleaves peptide bonds at proline residues. Upon cleavage by the target protease, H-L-Pro-pNA Trifluoracetate releases p-nitroaniline, a compound that exhibits a yellow color and can be quantified spectrophotometrically. This property allows researchers to monitor enzyme kinetics and to measure the activity of proteases in various biological samples. Moreover, it is instrumental in the screening of enzyme inhibitors, as changes in the rate of p-nitroaniline release reflect the inhibitory effects on the enzyme being studied.
Ordering Information
Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
H-L-Pro-pNA Trifluoracetate, 100 mg | sc-285986 | 100 mg | $100.00 | |||
H-L-Pro-pNA Trifluoracetate, 1 g | sc-285986A | 1 g | $129.00 |