Guanidine Hydrochloride CAS: 50-01-1
MF: CH5N3•HCl
MW: 95.53

Guanidine Hydrochloride (CAS 50-01-1)

Guanidine Hydrochloride | CAS 50-01-1 is rated 5.0 out of 5 by 2.
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Alternate Names: Guanidine Hydrochloride is also known as Aminoformamidine Hydrochloride
Application: Guanidine Hydrochloride is a widely used and powerful protein denaturant used in the purification of proteins and nucleic acids
CAS Number: 50-01-1
Purity: ≥99%
Molecular Weight: 95.53
Molecular Formula: CH5N3•HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids, especially mRNA. Guanidine Hydrochloride is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to be able to solubilize denatured insoluble proteins such as inclusion bodies. When utilized at lower concentrations, Guanidine Hydrochloride is noted to be useful in prompting the refolding of denatured proteins and had been reported to allow return of enzymatic activity in protocols using Guanidine Hydrochloride as a first step.


References

1. Pace, C.N., et al. 1990. Biochemistry. 29: 2564-2572. PMID: 2110472

2. Marston, F.A., et al. 1990. Meth. Enzymol. 182: 264-276. PMID: 2179665

3. Levine, A.D., et al. 1995. J. Biol. Chem. 270: 7445-7452. PMID: 7706290

4. Rudolph, R. and Lilie, H. 1996. FASEB J. 10: 49-56. PMID: 8566547

5. Mukhopadhyay, A. 1997. Adv. Biochem. Eng. Biotechnol. 56: 61-109. PMID: 8939059

Usage :
To make an 8M solution in water, heat the solution to 35°C for approximately 30 minutes. The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Note: Guanidine HCl may clump during storage. It may
appear as a free-flowing crystalline powder, a free-flowing powder with solid material dispersed throughout, or a solid. The quality of the product does
not appear to be affected and solutions prepared from
the free-flowing and lumpy guanidine HCl appear
identical.
Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in water (6 M) at 20° C, ethanol (300 g/l), and alcohol.
Storage :
Store at room temperature
Melting Point :
185-189° C
Density :
1.34 g/cm3 at 20° C
Refractive Index :
n20D 1.60 (Predicted)
IC50 :
Solute carrier family 22 member 2: IC50 = 171 µM (rat)
Ki Data :
Solute carrier family 22 member 2: Ki= 280 µM (rat); Solute carrier family 22 member 1: Ki= 724.2 µM (rat)
pK Values :
pKa: 13.6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
MF4300000
PubChem CID :
5742
Merck Index :
14: 4562
MDL Number :
MFCD00013026
EC Number :
200-002-3
Beilstein Registry :
3591990
SMILES :
C(=N)(N)N.Cl

Download SDS (MSDS)

Certificate of Analysis

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hola.........cuentan con algún distribuidor en México? para comprar 1 kg de hidrocloruro de guanidina gracias, saludos!!

Asked by: Gera
Thank you for your question. You can find the contact information for your distributor in Mexico here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-10-03

Hi I am trying to dissolve Guanidinium to 6M concentration (in DDW). its been 24h with stirrer but I am having no success to solubilize it totally! Could you suggest me what to do? Many thanks

Asked by: EsraaHaj
Thank you for your question. We suggest to try to heat the solution, or try 300g/L in ethanol.
Answered by: Technical Support
Date published: 2018-01-29
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Rated 5 out of 5 by from heat it heat it, companies heat it while trying to dissolve it.
Date published: 2017-09-13
Rated 5 out of 5 by from Various publications cite the use of Guanidine Various publications cite the use of Guanidine Hydrochloride for purification of proteins and nucleic acids. It is also useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies. -SCBT Publication Review
Date published: 2015-07-18
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