Molecular structure of Guanidine Hydrochloride, CAS Number: 50-01-1
Guanidine Hydrochloride (CAS 50-01-1)
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Alternate Names:
Guanidine Hydrochloride is also known as Aminoformamidine Hydrochloride
Application:
Guanidine Hydrochloride is a widely used and powerful protein denaturant used in the purification of proteins and nucleic acids
CAS Number:
50-01-1
Purity:
≥99%
Molecular Weight:
95.53
Molecular Formula:
CH5N3•HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids, especially mRNA. Furthermore, it is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to be able to solubilize denatured insoluble proteins such as inclusion bodies. When utilized at lower concentrations, Guanidine Hydrochloride is noted to be useful in prompting the refolding of denatured proteins and had been reported to allow return of enzymatic activity in protocols using Guanidine Hydrochloride as a first step.
Guanidine Hydrochloride (CAS 50-01-1) References
- Protein stabilization by urea and guanidine hydrochloride. | Bhuyan, AK. 2002. Biochemistry. 41: 13386-94. PMID: 12416983
- pH dependence of the urea and guanidine hydrochloride denaturation of ribonuclease A and ribonuclease T1. | Pace, CN., et al. 1990. Biochemistry. 29: 2564-72. PMID: 2110472
- Solubilization of protein aggregates. | Marston, FA. and Hartley, DL. 1990. Methods Enzymol. 182: 264-76. PMID: 2179665
- Effect of guanidine hydrochloride and urea on the interaction of 6-thioguanine with human serum albumin: a spectroscopic and molecular dynamics based study. | Ishtikhar, M., et al. 2016. J Biomol Struct Dyn. 34: 1409-20. PMID: 26208966
- Channel directed rutin nano-encapsulation in phytoferritin induced by guanidine hydrochloride. | Yang, R., et al. 2018. Food Chem. 240: 935-939. PMID: 28946364
- X-ray crystallographic analysis of time-dependent binding of guanidine hydrochloride to HEWL: First steps during protein unfolding. | Raskar, T., et al. 2019. Int J Biol Macromol. 122: 903-913. PMID: 30412756
- Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride. | Ngernna, S., et al. 2019. Malar J. 18: 148. PMID: 31023359
- Guanidine hydrochloride reactivates an ancient septin hetero-oligomer assembly pathway in budding yeast. | Johnson, CR., et al. 2020. Elife. 9: PMID: 31990274
- Unfolding and Aggregation of Lysozyme under the Combined Action of Dithiothreitol and Guanidine Hydrochloride: Optical Studies. | Sarimov, RM., et al. 2021. Int J Mol Sci. 22: PMID: 33800175
- Rapid and efficient microwave-assisted guanidine hydrochloride deep eutectic solvent pretreatment for biological conversion of castor stalk. | Zhong, L., et al. 2022. Bioresour Technol. 343: 126022. PMID: 34600092
- The role of guanidine hydrochloride in graphitic carbon nitride synthesis. | Smýkalová, A., et al. 2021. Sci Rep. 11: 21600. PMID: 34732765
- Protein denaturation with guanidine hydrochloride or urea provides a different estimate of stability depending on the contributions of electrostatic interactions. | Monera, OD., et al. 1994. Protein Sci. 3: 1984-91. PMID: 7703845
- High level expression and refolding of mouse interleukin 4 synthesized in Escherichia coli. | Levine, AD., et al. 1995. J Biol Chem. 270: 7445-52. PMID: 7706290
- In vitro folding of inclusion body proteins. | Rudolph, R. and Lilie, H. 1996. FASEB J. 10: 49-56. PMID: 8566547
- Inclusion bodies and purification of proteins in biologically active forms. | Mukhopadhyay, A. 1997. Adv Biochem Eng Biotechnol. 56: 61-109. PMID: 8939059
Inhibitor of:
α-S1-casein, γA-crystallin, Allophycocyanin, AMID, BDV, BFP, Bpifb9a, CAT-3, EBP1, FCP1, GFP, Hen Egg Lysozyme, KRTAP10-12, KRTAP4-13, KRTAP9-4, KRTAP9-8, Mumps, PNP2, Poliovirus Type 2, Poliovirus Type 3, RNase, RNase T2, Rotavirus, rotavirus C vp4, rotavirus C vp6, and rotavirus NSP4.Activator of:
γ-casein, 1110037F02Rik, 4732456N10Rik, AMPKγ3, ARP, ASCT1, BC014805, BPV E2, CD175s, Clpb, CPA2, CPD, cystatin SA, DDX11, DNase I, EF-1 β, eIF2β, HtrA, Importin-12, KDEL receptor 1, KDEL receptor 2, Keratin 74, Krtap31-1, KV3.1, Mengo 3DPol, Mesotrypsinogen, Poliovirus Type 1, prochymosin, Ribosomal Protein L31, Ribosomal Protein L37a, Ribosomal Protein L41, Ribosomal Protein L7L1, Ribosomal Protein LP2, Ribosomal Protein S12, RNase 1, RNase 12, Rnq1, Sar1, Sec31B, Ski2, SRP14, Sup35, TDRD6, TGaseZ, and TRAP-α.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Guanidine Hydrochloride, 100 g | sc-202637 | 100 g | $60.00 | |||
Guanidine Hydrochloride, 1 kg | sc-202637A | 1 kg | $195.00 |