Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids, especially mRNA. Guanidine Hydrochloride is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to be able to solubilize denatured insoluble proteins such as inclusion bodies. When utilized at lower concentrations, Guanidine Hydrochloride is noted to be useful in prompting the refolding of denatured proteins and had been reported to allow return of enzymatic activity in protocols using Guanidine Hydrochloride as a first step.
To make an 8M solution in water, heat the solution to 35°C for approximately 30 minutes. The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.
Note: Guanidine HCl may clump during storage. It may appear as a free-flowing crystalline powder, a free-flowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical.
Soluble in water (6 M) at 20° C, ethanol (300 g/l), and alcohol.
Store at room temperature
1.34 g/cm3 at 20° C
n20D 1.60 (Predicted)
Solute carrier family 22 member 2: IC50 = 171 µM (rat)
Solute carrier family 22 member 2: Ki= 280 µM (rat); Solute carrier family 22 member 1: Ki= 724.2 µM (rat)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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hola.........cuentan con algún distribuidor en México? para comprar 1 kg de hidrocloruro de guanidina
Asked by: Gera
Thank you for your question. You can find the contact information for your distributor in Mexico here:
Answered by: Tech Service
Date published: 2017-10-03
I am trying to dissolve Guanidinium to 6M concentration (in DDW). its been 24h with stirrer but I am having no success to solubilize it totally!
Could you suggest me what to do?
Asked by: EsraaHaj
Thank you for your question. We suggest to try to heat the solution, or try 300g/L in ethanol.
Rated 5 out of
mick highlanderson from
heat itheat it,
companies heat it while trying to dissolve it.
Date published: 2017-09-13
Rated 5 out of
Various publications cite the use of GuanidineVarious publications cite the use of Guanidine Hydrochloride for purification of proteins and nucleic acids. It is also useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies. -SCBT Publication Review
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