Gα z Double Nickase Plasmid (h): sc-402241-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gα z Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Gα z Double Nickase Plasmid (h) and Gα z Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GNAZ. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gα z Double Nickase Plasmid (h) | sc-402241-NIC | 20 µg | $410.00 | |||
Gα z Double Nickase Plasmid (h2) | sc-402241-NIC-2 | 20 µg | $410.00 |
GNAZ encodes the human Gαz subunit, a member of the inhibitory Gi/o family of heterotrimeric G proteins that couples activated GPCRs to downstream effectors. Gαz modulates cAMP signaling through regulation of adenylyl cyclase and influences MAPK and PI3K-associated pathways that shape cell excitability, secretion, and growth-related responses. As a pertussis toxin–insensitive G protein with distinct biochemical properties, it is studied in neuronal and endocrine signaling contexts where receptor-dependent second messenger dynamics are tightly controlled. Dysregulated GPCR–G protein signaling involving GNAZ has been investigated in disease-relevant models of aberrant proliferation and altered neurotransmission, supporting its utility as a mechanistic node in signaling network research.
Gα z Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GNAZ locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GNAZ. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GNAZ function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GNAZ-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.