Gα 14 Double Nickase Plasmid (h): sc-404556-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gα 14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Gα 14 Double Nickase Plasmid (h) and Gα 14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GNA14. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gα 14 Double Nickase Plasmid (h) | sc-404556-NIC | 20 µg | $410.00 | |||
Gα 14 Double Nickase Plasmid (h2) | sc-404556-NIC-2 | 20 µg | $410.00 |
GNA14 encodes the heterotrimeric G protein alpha subunit Gα14, a member of the Gq/11 family that couples activated GPCRs to phospholipase C-β. Through PLCβ-dependent generation of IP3 and diacylglycerol, Gα14 promotes intracellular Ca2+ mobilization and protein kinase C signaling, linking receptor inputs to MAPK/ERK activation and transcriptional programs that shape proliferation, differentiation, and secretory responses. GNA14 activity is particularly relevant in contexts where GPCR signaling governs vascular tone, endocrine function, and immune cell activation. Dysregulated Gq-class signaling, including alterations involving GNA14, has been implicated in aberrant growth signaling and tumor biology, supporting its use as a node to interrogate GPCR-driven pathways.
Gα 14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GNA14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GNA14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GNA14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GNA14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.