GSTT1 Double Nickase Plasmid (h): sc-408735-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSTT1 Double Nickase Plasmid (h) and GSTT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSTT1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTT1 Double Nickase Plasmid (h) | sc-408735-NIC | 20 µg | $410.00 | |||
GSTT1 Double Nickase Plasmid (h2) | sc-408735-NIC-2 | 20 µg | $410.00 |
Glutathione S-transferase theta 1 (GSTT1) is a phase II detoxification enzyme that catalyzes glutathione conjugation to electrophilic xenobiotics and reactive endogenous metabolites, supporting redox homeostasis and protection from oxidative stress. It contributes to cellular defense pathways that limit accumulation of DNA- and protein-damaging intermediates, influencing sensitivity to environmental toxicants and drug-derived reactive species. Common genetic variation and null genotypes in GSTT1 are associated with inter-individual differences in metabolism and have been studied in the context of cancer susceptibility, inflammatory disorders, and adverse responses to chemical exposures. As a cytosolic enzyme with broad substrate specificity, GSTT1 is frequently used to interrogate glutathione-dependent biotransformation and stress-response signaling in human cell models.
GSTT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSTT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSTT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSTT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSTT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.