GSTM2 Double Nickase Plasmid (h): sc-404795-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTM2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSTM2 Double Nickase Plasmid (h) and GSTM2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSTM2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSTM2 Antibody (E-9): sc-376486
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTM2 Double Nickase Plasmid (h) | sc-404795-NIC | 20 µg | $410.00 | |||
GSTM2 Double Nickase Plasmid (h2) | sc-404795-NIC-2 | 20 µg | $410.00 |
Glutathione S-transferase mu 2 (GSTM2) is a cytosolic phase II detoxification enzyme that catalyzes glutathione conjugation to electrophilic xenobiotics and endogenous lipid peroxidation products, supporting redox homeostasis and cellular defense against oxidative stress. As part of the glutathione metabolism network, GSTM2 contributes to the biotransformation of reactive intermediates and can influence cellular susceptibility to oxidative injury and inflammation-associated signaling. Variation in GSTM2 expression or activity has been linked to inter-individual differences in chemical sensitivity and stress responses, and it is frequently studied in contexts where reactive oxygen species and electrophile load shape disease-relevant phenotypes. In tumor biology and toxicology research, GSTM2 is also examined for its role in modulating responses to environmental exposures and drug metabolism pathways without implying clinical outcomes.
GSTM2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSTM2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSTM2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSTM2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSTM2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.