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GSA 10 is a smoothened (Smo) receptor agonist (EC50 = 1.2 μM). GSA 10 does not recognize the classic cyclopamine (sc-200929) binding site. It additonally does not promote Smo translocation to the primary cilium and is also strongly potentiated by cholera toxin and forskolin. GSA 10 also promotes differentiation of multipotent mesenchymal progenitor cells into osteoblasts.
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