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HSPA5/BiP/GRP78 Antibody (76-E6) - Western Blotting - Image 352723
GRP 78 抗体 (76-E6): sc-13539. A549 (A), SK-N-SH (B), Hep G2 (C), NIH/3T3 (D) 和 HeLa (E) 全细胞裂解液中 GRP 78 表达的 Western 印迹分析.
GRP 78 抗体 (76-E6): sc-13539. A549 (A), SK-N-SH (B), Hep G2 (C), NIH/3T3 (D) 和 HeLa (E) 全细胞裂解液中 GRP 78 表达的 Western 印迹分析.

HSPA5/BiP/GRP78 抗体 (76-E6): sc-13539

4.4(25)
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说明书
  • HSPA5/BiP/GRP78 抗体 76-E6 是大鼠单克隆 IgG，HSPA5/BiP/GRP78抗体, 在104篇文献中引用，规格为200 µg/ml
  • 针对全长GRP 78，mouse起源
  • HSPA5/BiP/GRP78 抗体 (76-E6) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 GRP 78
  • 抗HSPA5/BiP/GRP78抗体（76-E6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HSPA5/BiP/GRP78 (76-E6): sc-13539的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成HSPA5/BiP/GRP78 抗体 (76-E6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Glucose-Regulated Protein抗体，包括GRP 75, GRP 78和GRP 94

GRP 78抗体（76-E6）是一种IgG大鼠单克隆GRP 78抗体（也称为GRP78抗体、HSPA5抗体或BIP抗体），可通过WB和IP检测小鼠、大鼠和人源GRP 78蛋白。GRP 78抗体（76-E6）既可作为非偶联抗GRP 78抗体形式，也可作为多种偶联形式的抗GRP 78抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。HSP 70家族由四种高度保守的蛋白质组成：HSP 70、HSC 70、GRP 75和GRP 78。这些蛋白质具有多种作用：它们作为分子伴侣促进多蛋白复合物的组装，参与多肽跨细胞膜和细胞核的易位，并有助于新生多肽链的正确折叠。除HSP 70外，该家族的所有成员都在灵长类细胞中组成性表达。HSP 70的表达在热应激下被强烈诱导。HSP 70和HSC 70在胞质内质网和线粒体导入机制中发挥关键作用，并存在于哺乳动物细胞的胞质和细胞核中。HSP 70和HSC 70都参与新生多肽链的伴侣作用，并保护细胞免受错误折叠蛋白质的积累。GRP 78位于内质网中，在那里它接收输入的分泌蛋白，并参与新生肽链的折叠和易位。GRP 75的表达仅限于线粒体基质，并有助于核和线粒体来源的新生多肽链的易位和折叠。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSPA5/BiP/GRP78 抗体 (76-E6) 参考文献:

  1. 伴侣素 Hsp60 防止蛋白质在热应力下变性  |  Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
  2. 低温对小鼠 FM3A 细胞中 hsp70 mRNA 诱导及 hsp70 和 hsp105 积累的影响  |  Hatayama, T., et al. 1992. J Biochem. 111: 484-90. PMID: 1618738
  3. 克隆大鼠 grp75（一种 hsp70 家族成员）及其在正常和缺血性脑中的表达。  |  Massa, SM., et al. 1995. J Neurosci Res. 40: 807-19. PMID: 7629893
  4. 真核生物中蛋白质介导的蛋白质成熟。  |  Haas, IG. 1995. FEBS Lett. 369: 72-5. PMID: 7641888
  5. Hsp70 蛋白能充当产生力的马达吗？  |  Glick, BS. 1995. Cell. 80: 11-4. PMID: 7813006
  6. 人类线粒体 hsp70 的克隆和亚细胞定位。  |  Bhattacharyya, T., et al. 1995. J Biol Chem. 270: 1705-10. PMID: 7829505
  7. grp94（内质蛋白）的自身磷酸化。  |  Csermely, P., et al. 1995. J Biol Chem. 270: 6381-8. PMID: 7890776

订购信息

产品名称产品编号规格价格数量收藏夹

HSPA5/BiP/GRP78 抗体 (76-E6)

sc-13539
200 µg/ml
$316.00

HSPA5/BiP/GRP78 抗体 (76-E6) AC

sc-13539 AC
500 µg/ml, 25% agarose
$416.00

HSPA5/BiP/GRP78 抗体 (76-E6) HRP

sc-13539 HRP
200 µg/ml
$316.00

HSPA5/BiP/GRP78 抗体 (76-E6) FITC

sc-13539 FITC
200 µg/ml
$330.00

HSPA5/BiP/GRP78 抗体 (76-E6) PE

sc-13539 PE
200 µg/ml
$343.00

HSPA5/BiP/GRP78 抗体 (76-E6) Alexa Fluor® 488

sc-13539 AF488
200 µg/ml
$357.00

HSPA5/BiP/GRP78 抗体 (76-E6) Alexa Fluor® 546

sc-13539 AF546
200 µg/ml
$357.00

HSPA5/BiP/GRP78 抗体 (76-E6) Alexa Fluor® 594

sc-13539 AF594
200 µg/ml
$357.00

HSPA5/BiP/GRP78 抗体 (76-E6) Alexa Fluor® 647

sc-13539 AF647
200 µg/ml
$357.00

HSPA5/BiP/GRP78 抗体 (76-E6) Alexa Fluor® 680

sc-13539 AF680
200 µg/ml
$357.00

HSPA5/BiP/GRP78 抗体 (76-E6) Alexa Fluor® 790

sc-13539 AF790
200 µg/ml
$357.00

I see the western blot using this antibody gives a double band pattern at expected molecular weight. Are both the bands representing GRP78 and why is that? If only one of them is GRP78, which one is it?

Asked by: Phei000
Thank you for your question. Please contact technical service by emailing scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-08-13

Hello, Is the HSPA5/BiP/GRP78 Antibody (76-E6) sc-13539 raised against SBD of GRP78 (can you tell the sequence)? Is the epitope known for this antibody? Thank you!

Asked by: Ausra
Thank you for your question. HSPA5/BiP/GRP78 Antibody (76-E6): sc-13539 was raised against full length GRP 78 of mouse origin. No epitope mapping has been performed for this antibody as of yet, so its exact binding site is not known at this point.
Answered by: Tech Support Europe
Date published: 2023-10-10

Is this GRP 78(76-E6): sc-13539 antibody can use for IF?  I found it has been tested in some paper.

Asked by: wending
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-05-27

Hello. Is this antibody suitable for evaluation of expression of cell surface GRP78 and cytoplasmic GRP78? Thank you in advance!

Asked by: rrebelo
Thank you for your question. This antibody is not currently recommended for IF or IHC(P). We do not currently have any antigen localization data using this antibody. There are a few product citations that we are aware of using this antibody for IF or IHC. We recommend you review those references for any relevant information. For example, PMID's: 32184660, 30221722 and 31767864.
Answered by: Technical Support
Date published: 2020-07-09

Fiz um wester blot e apareceu duas bandas bem próximas em torno de 75 e 78 porque isso acontece?

Asked by: FRANCIELE
We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please reach out to your local distributor in Brazil or contact Technical Service directly. We are happy to help you optimize and troubleshoot the antibody to make sure you have success with the antibody.
Answered by: Tech Service
Date published: 2020-02-21

请问这个抗体的二抗是抗大鼠的二抗吗？

Asked by: yang200214yy
Thank you for your inquiry.GRP 78 Antibody (76-E6) is a rat monoclonal IgG.So you should use conjugated anti-rat secondary antibody. 感谢您的提问。GRP 78 (76-E6)是大鼠来源的单克隆抗体。您应该使用抗大鼠的二抗。
Answered by: Technical Support 9
Date published: 2019-11-27

Dear sir, I would like to apply for this antibody-(76-E6): sc-13539,but I don't know how to get it,would you please give me a help for applying for this? thank you

Asked by: Qinzi
Thank you for your question. For accurate availability, shipping and pricing information regarding our products, please contact Customer Service in China.
Answered by: Tech Service
Date published: 2019-05-07

I would like to use your GRP78 Ab conjugated with Alexa 647 for FACS analysis. I would like to know which Isotype do you recommend for your control. And do you have data showing the expression of GRP78 by FACS with your AB?

Asked by: DeborahM
Thank you for your inquiry and your interest in this product. As isotype control we would suggest to use our sc-516643 normal rat IgG Alexa Fluor® 647. The GRP 78 Antibody (76-E6): sc-13539 is recommended for detection of GRP 78 of mouse, rat and human origin by WB and IP. We have not yet tested this antibody in flow cytometry. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Support Europe
Date published: 2017-12-29
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Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug of protein sample. Ab dilution will be 1:200 for overnight @ 4oC.
Date published: 2024-12-10
Rated 1 out of 5 by from Concern about antibody specilificyWe used this antibody on a clean membrane and received a single band. However, this band is not at the expected size of GRP78 but significantly lower. We received this result in two cell lines with endogenous expression of GRP78 (HEK 293 and HCT-116).
Date published: 2024-06-27
Rated 1 out of 5 by from The blot comes out below the molecular weightDetection by wb in HEK-293 resulted in a band below 70 kDa
Date published: 2024-02-21
Rated 5 out of 5 by from Good result!I used this Ab (1:500, 16 h 4 °C) to blot the GRP78 in SH-SY5Y cells (30 ug total lysate), secondary antibody was mouse (1:1000, 1 h room temp). The band was very clear and strong at 70-75 kDa.
Date published: 2023-03-23
Rated 5 out of 5 by from 条带清晰本次的实验条带还是很清晰的，已经发表了一篇文章，下次还会继续支持购买。许愿有个好的实验结果！！！！！
Date published: 2022-11-01
Rated 3 out of 5 by from Be aware of the target!This antibody also targets HSP70! So please interpret your data carefully!!
Date published: 2020-08-23
Rated 5 out of 5 by from WB resultWe used this Ab (1:800) to blot the BIP in HepG2 cells. The band was very clear and strong.
Date published: 2020-08-22
Rated 5 out of 5 by from Ottimo anticorpoTestato su lisato piastrinico. Segnale davvero bello e pulito! visibile facilmente con 50 ug di lisato. Da fare attenzione solo al MW, leggermente più basso di 78 KDa.
Date published: 2020-07-30
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HSPA5/BiP/GRP78 抗体 (76-E6) is rated 4.4 out of 5 by 25.
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