group VI iPLA2 Double Nickase Plasmid (h): sc-402107-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- group VI iPLA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- group VI iPLA2 Double Nickase Plasmid (h) and group VI iPLA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PLA2G6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: group VI iPLA2 Antibody (D-4): sc-376563
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
group VI iPLA2 Double Nickase Plasmid (h) | sc-402107-NIC | 20 µg | $410.00 | |||
group VI iPLA2 Double Nickase Plasmid (h2) | sc-402107-NIC-2 | 20 µg | $410.00 |
PLA2G6 encodes group VI iPLA2, a calcium-independent phospholipase A2 that hydrolyzes membrane phospholipids to generate free fatty acids and lysophospholipids, shaping lipid signaling and membrane remodeling. This enzyme contributes to phospholipid homeostasis, mitochondrial integrity, vesicular trafficking, and inflammatory mediator biology through arachidonic acid–linked pathways. PLA2G6 activity intersects with oxidative stress responses and programmed cell death, influencing cellular susceptibility to lipid peroxidation and organelle dysfunction. Genetic perturbations in PLA2G6 are linked to neurodegeneration with brain iron accumulation and related early-onset parkinsonian phenotypes, supporting its relevance to neuronal maintenance and lipid metabolism research.
group VI iPLA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PLA2G6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PLA2G6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PLA2G6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PLA2G6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.