Date published: 2025-10-16

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group II sPLA2 Antibody (SCACC353): sc-58363

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Datasheets
  • group II sPLA2 Antibody (SCACC353) is a mouse monoclonal IgG1 κ group II sPLA2 antibody, cited in 4 publications, provided at 100 µg/ml
  • raised against a recombinant protein representing full-length group II sPLA2 of human origin
  • recommended for detection of group II sPLA2 of human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with type V sPLA2
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for group II sPLA2 Antibody (SCACC353). This work is in progress.

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Group II sPLA2 Antibody (SCACC353) is a mouse monoclonal IgG1 kappa light chain antibody that detects group II sPLA2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-group II sPLA2 antibody (SCACC353) is available as a non-conjugated format. Group II sPLA2 (SCACC353) antibody recognizes a protein that plays a crucial role in phospholipid hydrolysis, leading to fatty acid release essential for membrane remodeling and signaling pathways. Group II sPLA2 (SCACC353) monoclonal antibody targets an enzyme important in inflammatory responses, generating bioactive lipids that modulate immune cell function. Group II sPLA2 protein interacts with cell membranes, influencing phospholipid metabolism and eicosanoid production, which are signaling molecules involved in inflammation and other physiological responses. The human gene encoding group II sPLA2 is located on chromosome 1, with expression tightly regulated in various tissues, highlighting significance in both normal physiology and pathological conditions.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

group II sPLA2 Antibody (SCACC353) References:

  1. Human pancreatic islets express mRNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on chromosome 22q13.1.  |  Ma, Z., et al. 1999. J Biol Chem. 274: 9607-16. PMID: 10092647
  2. The human calcium-independent phospholipase A2 gene multiple enzymes with distinct properties from a single gene.  |  Larsson Forsell, PK., et al. 1999. Eur J Biochem. 262: 575-85. PMID: 10336645
  3. Purification of recombinant human secretory phospholipase A2 (group II) produced in long-term immobilized cell culture.  |  Levin, W., et al. 1992. Protein Expr Purif. 3: 27-35. PMID: 1422206
  4. Natural disruption of group 2 phospholipase A2 gene protects against choline-deficient ethionine-supplemented diet-induced acute pancreatitis and lung injury.  |  Kihara, Y., et al. 2005. Pancreas. 31: 48-53. PMID: 15968247
  5. The role of phospholipases A2 in schizophrenia.  |  Law, MH., et al. 2006. Mol Psychiatry. 11: 547-56. PMID: 16585943
  6. Structures of free and inhibited human secretory phospholipase A2 from inflammatory exudate.  |  Scott, DL., et al. 1991. Science. 254: 1007-10. PMID: 1948070
  7. Inhibition of sPLA2 and endothelial function: a substudy of the SPIDER-PCI trial.  |  Lavi, S., et al. 2012. Can J Cardiol. 28: 215-21. PMID: 22281412
  8. Role of secretory phospholipase A₂ in women with metabolic syndrome.  |  Pop, D., et al. 2013. Indian J Med Res. 138: 866-72. PMID: 24521628
  9. Protective effect of quinacrine against glycerol-induced acute kidney injury in rats.  |  Al Asmari, AK., et al. 2017. BMC Nephrol. 18: 41. PMID: 28129740
  10. Secretion of group 2 phospholipase A2 by lacrimal glands.  |  Nevalainen, TJ., et al. 1994. Invest Ophthalmol Vis Sci. 35: 417-21. PMID: 8112989
  11. Cloning, chromosomal mapping, and expression of a novel human secretory phospholipase A2.  |  Cupillard, L., et al. 1997. J Biol Chem. 272: 15745-52. PMID: 9188469
  12. Crystal structure of human secretory phospholipase A2-IIA complex with the potent indolizine inhibitor 120-1032.  |  Kitadokoro, K., et al. 1998. J Biochem. 123: 619-23. PMID: 9538252

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

group II sPLA2 Antibody (SCACC353)

sc-58363
100 µg/ml
$316.00

Can sc-58363: group II sPLA2 (SCACC353) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, sc-58363: group II sPLA2 (SCACC353) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with mouse Distal ileum tissue extract in published research -SCBT QC
Date published: 2023-09-14
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group II sPLA 2 Antibody (SCACC353) is rated 3.5 out of 5 by 2.
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