Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GROβ CRISPR Activation Plasmid (h): sc-416416-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GROβ CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- GROβ CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by GROβ CRISPR Activation Plasmid (h) and GROβ CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CXCL2 transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GROβ CRISPR Activation Plasmid (h) | sc-416416-ACT | 20 µg | $397.00 |
CXCL2 encodes growth-regulated oncogene beta (GROβ), a CXC chemokine that signals primarily through CXCR2 to promote neutrophil chemotaxis, adhesion, and activation during acute inflammatory responses. GROβ expression is induced by NF-κB and MAPK pathway activity downstream of pattern-recognition receptors and pro-inflammatory cytokines, linking innate immune sensing to leukocyte recruitment. In tissue microenvironments, CXCL2 contributes to cytokine and chemokine network amplification that shapes myeloid cell trafficking, angiogenic cues, and stromal–immune crosstalk. Dysregulated CXCL2/GROβ signaling is frequently studied in inflammatory disorders and in tumor-associated inflammation where neutrophil and myeloid infiltration can influence disease-associated phenotypes.
GROβ CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CXCL2 expression without altering the underlying DNA sequence.
GROβ CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CXCL2 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CXCL2 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous GROβ expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CXCL2 locus and enabling the study of GROβ-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of GROβ pathway restoration in tumor cells with silenced or reduced CXCL2 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.