GRIP1 Antibody (H-4): sc-365937

GRIP1 Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects GRIP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-GRIP1 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GRIP1 plays a crucial role in synaptic transmission and plasticity by interacting with AMPA receptor subunits, specifically GluR-2 and GluR-3, which are essential for mediating excitatory neurotransmission in the brain. This interaction is vital for proper targeting of these receptors to the synapse, influencing neural development and mechanisms underlying neurodegeneration. GRIP1 is predominantly expressed in the cerebral cortex, hippocampus, and olfactory bulb, with significant enrichment in synaptic plasma and postsynaptic density fractions. GRIP1 is expressed during early development, preceding the expression of AMPA receptors, and is involved in the regulation of receptor endocytosis in response to phosphorylation events, such as those mediated by protein kinase C. This regulation is particularly important for processes like cerebellar long-term depression, highlighting GRIP1′s significance in both normal brain function and potential pathological conditions.

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GRIP1 Antibody (H-4) References:

1. Association of AMPA receptors with a subset of glutamate receptor-interacting protein in vivo.  |  Wyszynski, M., et al. 1999. J Neurosci. 19: 6528-37. PMID: 10414981
2. Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2.  |  Dong, H., et al. 1999. J Neurosci. 19: 6930-41. PMID: 10436050
3. Disruption of AMPA receptor GluR2 clusters following long-term depression induction in cerebellar Purkinje neurons.  |  Matsuda, S., et al. 2000. EMBO J. 19: 2765-74. PMID: 10856222
4. Mutagenesis reveals a role for ABP/GRIP binding to GluR2 in synaptic surface accumulation of the AMPA receptor.  |  Osten, P., et al. 2000. Neuron. 27: 313-25. PMID: 10985351
5. Cerebellar long-term depression requires PKC-regulated interactions between GluR2/3 and PDZ domain-containing proteins.  |  Xia, J., et al. 2000. Neuron. 28: 499-510. PMID: 11144359
6. Differential palmitoylation of two mouse glutamate receptor interacting protein 1 forms with different N-terminal sequences.  |  Yamazaki, M., et al. 2001. Neurosci Lett. 304: 81-4. PMID: 11335060
7. Review of the in vivo functions of the p160 steroid receptor coactivator family.  |  Xu, J. and Li, Q. 2003. Mol Endocrinol. 17: 1681-92. PMID: 12805412
8. Molecular diversity of glutamate receptors and implications for brain function.  |  Nakanishi, S. 1992. Science. 258: 597-603. PMID: 1329206
9. Molecular mechanisms involved in the growth stimulation of breast cancer cells by leptin.  |  Yin, N., et al. 2004. Cancer Res. 64: 5870-5. PMID: 15313931
10. Recruitment of coactivator glucocorticoid receptor interacting protein 1 to an estrogen receptor transcription complex is regulated by the 3',5'-cyclic adenosine 5'-monophosphate-dependent protein kinase.  |  Fenne, IS., et al. 2008. Endocrinology. 149: 4336-45. PMID: 18499756
11. Steroid receptor coactivator 2: an essential coregulator of progestin-induced uterine and mammary morphogenesis.  |  Mukherjee, A., et al. 2007. Ernst Schering Found Symp Proc. 55-76. PMID: 18540568
12. The role of glutamate neurotoxicity in hypoxic-ischemic neuronal death.  |  Choi, DW. and Rothman, SM. 1990. Annu Rev Neurosci. 13: 171-82. PMID: 1970230

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GRIP1 Antibody (H-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-GRIP1 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GRIP1 Antibody (H-4) Specifications

Alternate Product Name: GRIP1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 910-1100

Epitope Target: GRIP1

Epitope Species: rat

Product Reactivity/Detection: GRIP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for GRIP1 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin