GRAP1 Antibody (A-6): sc-398198

GRAP1 Antibody (A-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects GRAP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRAP1 (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GRAP1, also known as GRASP-1 or GRIP-associated protein, plays a crucial role in cellular signaling as a member of the rasGEF (Ras protein GDP/GTP exchange factors) family, which is essential for the regulation of Ras signaling pathways. GRAP1 contains a unique structural arrangement featuring a rasGEF domain, a caspase-3 cleavage site, a region homologous to the ras binding domain, and a PDZ domain. This structural configuration is significant because the caspase-3 cleavage site divides GRAP1 into an amino-terminal GEF catalytic domain and a carboxyl-terminal regulatory domain, which is a proteolytic fragment. The presence of these domains allows GRAP1 to interact with other proteins, such as the seventh PDZ domain of GRIP-1, which binds to the C termini of AMPA receptors, potentially linking them to other signaling molecules. GRAP1 is predominantly expressed in the cytosol and is partially localized to the membrane in all tissues of the nervous system, while the cleaved fragment remains in the cytosol. The regulation of GRAP1 by caspase-3 is particularly important, as this process may disrupt the proper targeting and function of the GEF, thereby influencing the distribution of AMPA receptors and the overall signaling dynamics within neuronal cells.

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GRAP1 Antibody (A-6) References:

1. Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2.  |  Dong, H., et al. 1999. J Neurosci. 19: 6930-41. PMID: 10436050
2. GRASP-1: a neuronal RasGEF associated with the AMPA receptor/GRIP complex.  |  Ye, B., et al. 2000. Neuron. 26: 603-17. PMID: 10896157
3. Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.  |  Goodarzi, H., et al. 2016. Cell. 165: 1416-1427. PMID: 27259150
4. A novel RBMX-TFE3 gene fusion in a highly aggressive pediatric renal perivascular epithelioid cell tumor.  |  Argani, P., et al. 2020. Genes Chromosomes Cancer. 59: 58-63. PMID: 31408245
5. Analysis of deubiquitinase OTUD5 as a biomarker and therapeutic target for cervical cancer by bioinformatic analysis.  |  Bai, M., et al. 2020. PeerJ. 8: e9146. PMID: 32655987
6. X Chromosome Contribution to the Genetic Architecture of Primary Biliary Cholangitis.  |  Asselta, R., et al. 2021. Gastroenterology. 160: 2483-2495.e26. PMID: 33675743
7. Profiling Analysis of Circular RNA and mRNA in Human Temporal Lobe Epilepsy with Hippocampal Sclerosis ILAE Type 1.  |  Gu, Y., et al. 2022. Cell Mol Neurobiol. 42: 2745-2755. PMID: 34338959
8. Analysis of Expression of the GRIPAP1, DLG4, KIF1B, NGFRAP1, and NRF1 Genes in Peripheral Blood of the Patients with Parkinson's Disease in the Early Clinical Stages.  |  Lukashevich, MV., et al. 2024. Biochemistry (Mosc). 89: 1779-1788. PMID: 39523115
9. Identification of the guanine nucleotide dissociation stimulator for Ral as a putative effector molecule of R-ras, H-ras, K-ras, and Rap.  |  Spaargaren, M. and Bischoff, JR. 1994. Proc Natl Acad Sci U S A. 91: 12609-13. PMID: 7809086
10. ralGDS family members interact with the effector loop of ras p21.  |  Kikuchi, A., et al. 1994. Mol Cell Biol. 14: 7483-91. PMID: 7935463
11. GRIP: a synaptic PDZ domain-containing protein that interacts with AMPA receptors.  |  Dong, H., et al. 1997. Nature. 386: 279-84. PMID: 9069286
12. Novel anchorage of GluR2/3 to the postsynaptic density by the AMPA receptor-binding protein ABP.  |  Srivastava, S., et al. 1998. Neuron. 21: 581-91. PMID: 9768844

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GRAP1 Antibody (A-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-GRAP1 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GRAP1 Antibody (A-6) Specifications

Alternate Product Name: GRAP1

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 375-483

Epitope Target: GRAP1

Epitope Species: human

Product Reactivity/Detection: GRAP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for GRAP1 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin