granulysin Antibody (F-9): sc-271119

granulysin Antibody (F-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects granulysin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). granulysin Antibody (F-9) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. granulysin, also known as NKG5, is a saposin-like protein (SAPLIP) predominantly expressed in cytotoxic lymphocytes, where granulysin plays a crucial role in immune response against intracellular pathogens. granulysin mediates the release of cytotoxic granules from cytolytic T lymphocytes (CTLs) and natural killer (NK) cells upon stimulation by antigens from bacteria, fungi, and parasites. granulysin′s mechanism of action involves increasing the permeability of pathogen host cells, leading to their lysis and subsequent apoptosis. granulysin is produced in two distinct sizes from three unique transcripts, highlighting complex regulation. granulysin-expressing CTLs, in conjunction with perforin, can effectively target and eliminate both extracellular and intracellular Mycobacterium tuberculosis, underscoring granulysin′s significant role in T cell-mediated immunity and host protection against various pathogens.

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granulysin Antibody (F-9) References:

1. Biosynthesis of granulysin, a novel cytolytic molecule.  |  Hanson, DA., et al. 1999. Mol Immunol. 36: 413-22. PMID: 10449094
2. Granulysin: a lethal weapon of cytolytic T cells.  |  Stenger, S., et al. 1999. Immunol Today. 20: 390-4. PMID: 10462738
3. Granulysin expression increases with increasing clinical severity of psoriasis.  |  Elgarhy, LH., et al. 2015. Clin Exp Dermatol. 40: 361-6. PMID: 25639185
4. Death ligands and granulysin: mechanisms of tumor cell death induction and therapeutic opportunities.  |  Martinez-Lostao, L., et al. 2015. Immunotherapy. 7: 883-2. PMID: 26314314
5. Granulysin, a novel marker for extranodal NK/T cell lymphoma, nasal type.  |  Lo Bello, G., et al. 2018. Virchows Arch. 473: 749-757. PMID: 30151671
6. Decidual NK Cells Transfer Granulysin to Selectively Kill Bacteria in Trophoblasts.  |  Crespo, ÂC., et al. 2020. Cell. 182: 1125-1139.e18. PMID: 32822574
7. Use of the Human Granulysin Transgenic Mice To Evaluate the Role of Granulysin Expression by CD8 T Cells in Immunity To Mycobacterium tuberculosis.  |  Thakur, P., et al. 2022. mBio. 13: e0302022. PMID: 36409085
8. Involvement of M1-Activated Macrophages and Perforin/Granulysin Expressing Lymphocytes in IgA Vasculitis Nephritis.  |  Laskarin, G., et al. 2024. Int J Mol Sci. 25: PMID: 38396930
9. Genomic structure of NKG5, a human NK and T cell-specific activation gene.  |  Houchins, JP., et al. 1993. Immunogenetics. 37: 102-7. PMID: 8423048
10. Granulysin, a new human cytolytic granule-associated protein with possible involvement in cell-mediated cytotoxicity.  |  Peña, SV. and Krensky, AM. 1997. Semin Immunol. 9: 117-25. PMID: 9194222
11. Granulysin-induced apoptosis. I. Involvement of at least two distinct pathways.  |  Gamen, S., et al. 1998. J Immunol. 161: 1758-64. PMID: 9712041
12. An antimicrobial activity of cytolytic T cells mediated by granulysin.  |  Stenger, S., et al. 1998. Science. 282: 121-5. PMID: 9756476

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-granulysin Antibody (F-9) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-granulysin Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

granulysin Antibody (F-9) Specifications

Alternate Product Name: granulysin

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 16-145

Epitope Target: mature granulysin

Epitope Species: human

Product Reactivity/Detection: precursor and mature forms of granulysin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for granulysin Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin