Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPR56 Double Nickase Plasmid (h): sc-406370-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR56 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR56 Double Nickase Plasmid (h) and GPR56 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADGRG1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPR56 Antibody (G-6): sc-390192
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR56 Double Nickase Plasmid (h) | sc-406370-NIC | 20 µg | $410.00 | |||
GPR56 Double Nickase Plasmid (h2) | sc-406370-NIC-2 | 20 µg | $410.00 |
ADGRG1 encodes GPR56, an adhesion G protein–coupled receptor enriched in neural and immune lineages that integrates extracellular matrix cues with intracellular signaling. GPR56 participates in cell–cell and cell–matrix interactions, modulating processes such as neuronal migration, cortical development, and cytoskeletal organization through GPCR-linked pathways including RhoA-driven actin dynamics. In hematopoietic contexts, GPR56 has been implicated in regulating adhesion, trafficking, and functional states of leukocyte subsets, supporting investigation of immune cell positioning and activation programs. Dysregulated ADGRG1/GPR56 activity has been associated with neurodevelopmental disorders and altered tumor cell behavior, making it a relevant target for mechanistic studies of adhesion signaling and lineage-specific differentiation.
GPR56 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADGRG1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADGRG1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADGRG1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADGRG1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.