Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPR43 Double Nickase Plasmid (h): sc-401858-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR43 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR43 Double Nickase Plasmid (h) and GPR43 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FFAR2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPR43 Antibody (3B3): sc-293202
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR43 Double Nickase Plasmid (h) | sc-401858-NIC | 20 µg | $410.00 | |||
GPR43 Double Nickase Plasmid (h2) | sc-401858-NIC-2 | 20 µg | $410.00 |
Human FFAR2 encodes GPR43, a short-chain fatty acid–sensing GPCR that is activated by acetate and propionate and couples primarily to Gαi/o and Gαq signaling. GPR43 regulates cAMP suppression, PLC-dependent calcium mobilization, and downstream MAPK and NF-κB-linked programs that shape chemotaxis, cytokine release, and barrier-related immune responses. Expression in myeloid lineages and epithelial contexts links FFAR2 to microbial metabolite sensing and metabolic–inflammatory crosstalk. Dysregulated GPR43 signaling has been associated with altered leukocyte recruitment and inflammatory phenotypes relevant to gastrointestinal inflammation, airway inflammation, and metabolic dysfunction.
GPR43 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FFAR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FFAR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FFAR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FFAR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.