GPR19 Double Nickase Plasmid (h): sc-407000-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR19 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR19 Double Nickase Plasmid (h) and GPR19 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPR19. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR19 Double Nickase Plasmid (h) | sc-407000-NIC | 20 µg | $410.00 | |||
GPR19 Double Nickase Plasmid (h2) | sc-407000-NIC-2 | 20 µg | $410.00 |
GPR19 encodes an orphan class A G protein-coupled receptor that is predominantly localized to intracellular membranes and has been implicated in regulation of cyclic nucleotide signaling, cell survival programs, and neuronal/endocrine homeostasis. Reported functional studies link GPR19 to modulation of MAPK/ERK and cAMP-dependent pathways, suggesting roles in stimulus–response coupling and transcriptional control downstream of GPCR signaling. Expression patterns and genetic/omic associations have connected GPR19 to neurobehavioral phenotypes and neurodegenerative processes, including correlations described in Parkinson’s disease–related datasets. As a membrane signaling node with incompletely defined ligands and downstream effectors, GPR19 is of interest for dissecting GPCR pathway wiring and context-dependent signaling in human cell models.
GPR19 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPR19 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPR19. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPR19 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPR19-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.