GPR17 Double Nickase Plasmid (h): sc-402977-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR17 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR17 Double Nickase Plasmid (h) and GPR17 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPR17. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPR17 Antibody (A-10): sc-514723
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR17 Double Nickase Plasmid (h) | sc-402977-NIC | 20 µg | $410.00 | |||
GPR17 Double Nickase Plasmid (h2) | sc-402977-NIC-2 | 20 µg | $410.00 |
GPR17 encodes a rhodopsin-like G protein-coupled receptor that integrates extracellular purinergic and cysteinyl leukotriene–related signals to modulate intracellular second-messenger pathways, including cAMP and calcium-dependent signaling. In the central nervous system, GPR17 expression is enriched in oligodendrocyte lineage cells and is implicated in coordinating the timing of differentiation and myelination programs, linking receptor activity to neuroinflammatory cues and tissue remodeling. The receptor has also been studied in contexts of ischemic stress responses and inflammatory signaling, where altered GPCR signaling can reshape glial activation states and downstream transcriptional networks. As a result, GPR17 is a useful target for dissecting GPCR-driven regulation of neural development, inflammation-associated signaling, and cell-fate transitions.
GPR17 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPR17 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPR17. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPR17 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPR17-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.