GPR126 CRISPR/Cas9 KO Plasmid (h): sc-407085
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR126 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR126 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR126 CRISPR/Cas9 KO Plasmid (h) | sc-407085 | 20 µg | $397.00 |
Overview
ADGRG6 (GPR126) encodes an adhesion G protein–coupled receptor that integrates extracellular matrix and cell–cell cues to regulate signaling through heterotrimeric G proteins, including cAMP/PKA-dependent pathways. GPR126 is a key regulator of Schwann cell development and myelination and contributes to peripheral nerve maturation, with additional roles in cartilage biology, skeletal growth, and vascular development. Through its large extracellular domain and autoproteolysis at the GPCR proteolysis site, GPR126 influences mechanosensitive and developmental signaling programs. Genetic and functional perturbations of ADGRG6 have been associated with neurodevelopmental and musculoskeletal phenotypes, supporting its relevance for studying myelination, cell differentiation, and tissue morphogenesis.
GPR126 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ADGRG6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADGRG6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADGRG6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR126 protein expression.
This CRISPR knockout system enables efficient generation of ADGRG6-deficient cell models for investigation of GPR126 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ADGRG6 exon(s) critical for GPR126 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ADGRG6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR126 CRISPR/Cas9 KO Plasmid (h) and GPR126 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADGRG6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR126 HDR Plasmid (h) and GPR126 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADGRG6 homology arms to support homology-directed repair at defined ADGRG6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.