GPR125 Double Nickase Plasmid (h): sc-408335-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR125 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR125 Double Nickase Plasmid (h) and GPR125 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADGRA3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR125 Double Nickase Plasmid (h) | sc-408335-NIC | 20 µg | $410.00 | |||
GPR125 Double Nickase Plasmid (h2) | sc-408335-NIC-2 | 20 µg | $410.00 |
ADGRA3 encodes the adhesion G protein–coupled receptor GPR125, a multi-pass membrane receptor implicated in cell–cell and cell–matrix interactions and in modulation of intracellular signaling through GPCR-associated pathways. As an adhesion GPCR, GPR125 is linked to regulation of cellular polarity, migration, and differentiation programs, with reported roles in epithelial and stem/progenitor cell biology. Altered ADGRA3/GPR125 expression has been studied in the context of tumor biology and tissue remodeling, where changes in adhesion-linked signaling can influence invasive behavior and microenvironmental responses. These features make ADGRA3 a useful target for dissecting adhesion-dependent signaling networks and lineage-associated transcriptional programs in human cell models.
GPR125 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADGRA3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADGRA3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADGRA3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADGRA3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.