GPI Double Nickase Plasmid (h): sc-402796-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPI Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPI Double Nickase Plasmid (h) and GPI Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPI. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPI Antibody (H-10): sc-365066
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPI Double Nickase Plasmid (h) | sc-402796-NIC | 20 µg | $410.00 | |||
GPI Double Nickase Plasmid (h2) | sc-402796-NIC-2 | 20 µg | $410.00 |
Glucose-6-phosphate isomerase (GPI) is a cytosolic enzyme that catalyzes the reversible interconversion of glucose-6-phosphate and fructose-6-phosphate, linking glycolysis and gluconeogenesis and influencing cellular energy balance. By shaping carbon flux through central metabolism, GPI can affect redox homeostasis and biosynthetic precursor availability in proliferative and stress-adapted cells. Beyond its canonical enzymatic role, GPI has been implicated in extracellular signaling activities that can modulate cell motility and microenvironmental interactions, depending on context. Genetic or functional perturbation of GPI is associated with metabolic dysregulation and has been studied in relation to anemia and neurodevelopmental phenotypes as well as metabolic features observed in cancer models.
GPI Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPI locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPI. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPI function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPI-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.