GPI Antibody (H-10): sc-365066

GPI Antibody (H-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects GPI protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GPI antibody (H-10) is available as the non-conjugated form. Glucose-6-phosphate isomerase (GPI), also known as Phosphohexose isomerase (PHI), Neuroleukin (NLK), and Spermantigen-36 (SA-36), plays a crucial role in cellular metabolism by catalyzing the reversible conversion of glucose-6-phosphate to fructose-6-phosphate, a key step in both glycolysis and gluconeogenesis. This enzymatic function maintains energy homeostasis in cells, particularly in tissues with high metabolic demands, such as the brain and muscles. GPI also acts as a neurotrophic factor, supporting the survival and growth of spinal and sensory neurons, which underscores GPI′s importance in neurodevelopment and neuroprotection. Defects or mutations in the GPI gene can lead to serious health issues, including hereditary nonspherocytic hemolytic anemia, hydrops fetalis, immediate neonatal death, and neurological impairments, highlighting GPI′s critical nature in both metabolic and neurological contexts.

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GPI Antibody (H-10) References:

1. Glucose-6-phosphate isomerase is not a specific autoantigen in rheumatoid arthritis.  |  Herve, CA., et al. 2003. Rheumatology (Oxford). 42: 986-8. PMID: 12730513
2. Glucose-6-phosphate isomerase is necessary for embryo implantation in the domestic ferret.  |  Schulz, LC. and Bahr, JM. 2003. Proc Natl Acad Sci U S A. 100: 8561-6. PMID: 12826606
3. Detection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore).  |  Kim, JY., et al. 2003. Exp Mol Med. 35: 310-6. PMID: 14508072
4. Immunization with glucose-6-phosphate isomerase induces T cell-dependent peripheral polyarthritis in genetically unaltered mice.  |  Schubert, D., et al. 2004. J Immunol. 172: 4503-9. PMID: 15034067
5. The crystal structure of mouse phosphoglucose isomerase at 1.6A resolution and its complex with glucose 6-phosphate reveals the catalytic mechanism of sugar ring opening.  |  Solomons, JT., et al. 2004. J Mol Biol. 342: 847-60. PMID: 15342241
6. Glucose-6-phosphate isomerase variants play a key role in the generation of anti-GPI antibodies: possible mechanism of autoantibody production.  |  Muraki, Y., et al. 2004. Biochem Biophys Res Commun. 323: 518-22. PMID: 15369782
7. Raised levels of anti-glucose-6-phosphate isomerase IgG in serum and synovial fluid from patients with inflammatory arthritis.  |  Schaller, M., et al. 2005. Ann Rheum Dis. 64: 743-9. PMID: 15458959
8. Elevated serum glucose-6-phosphate isomerase correlates with histological disease activity and clinical improvement after initiation of therapy in patients with rheumatoid arthritis.  |  Dai, L., et al. 2010. J Rheumatol. 37: 2452-61. PMID: 20810510
9. Peptidylarginine deiminase type 4 deficiency reduced arthritis severity in a glucose-6-phosphate isomerase-induced arthritis model.  |  Seri, Y., et al. 2015. Sci Rep. 5: 13041. PMID: 26293116
10. Two novel mutations (p.(Ser160Pro) and p.(Arg472Cys)) causing glucose-6-phosphate isomerase deficiency are associated with erythroid dysplasia and inappropriately suppressed hepcidin.  |  Mojzikova, R., et al. 2018. Blood Cells Mol Dis. 69: 23-29. PMID: 28803808
11. Glucosephosphate isomerase (GPI) deficiency mutations associated with hereditary nonspherocytic hemolytic anemia (HNSHA).  |  Beutler, E., et al. 1997. Blood Cells Mol Dis. 23: 402-9. PMID: 9446754
12. Molecular basis of neurological dysfunction coupled with haemolytic anaemia in human glucose-6-phosphate isomerase (GPI) deficiency.  |  Kugler, W., et al. 1998. Hum Genet. 103: 450-4. PMID: 9856489

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GPI Antibody (H-10) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-GPI Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GPI Antibody (H-10) Specifications

Alternate Product Name: GPI

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 67-102

Epitope Target: GPI

Epitope Species: human

Product Reactivity/Detection: GPI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for GPI Antibody (H-10): P: Blocking Peptide