GPD1 Double Nickase Plasmid (h): sc-403706-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPD1 Double Nickase Plasmid (h) and GPD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPD1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPD1 Antibody (E-7): sc-376219
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPD1 Double Nickase Plasmid (h) | sc-403706-NIC | 20 µg | $410.00 | |||
GPD1 Double Nickase Plasmid (h2) | sc-403706-NIC-2 | 20 µg | $410.00 |
Human GPD1 encodes cytosolic glycerol-3-phosphate dehydrogenase 1, a NADH-dependent enzyme that interconverts dihydroxyacetone phosphate and glycerol-3-phosphate, linking glycolysis to glycerolipid synthesis and redox homeostasis. By contributing to the glycerol phosphate shuttle, GPD1 influences cellular NADH/NAD+ balance, mitochondrial electron transfer, and lipid metabolic flux under varying nutrient conditions. Altered GPD1 activity has been associated with metabolic phenotypes that involve triglyceride handling, insulin-responsive pathways, and hepatic lipid accumulation, making it relevant to studies of adipose and liver biology. As a node connecting carbohydrate utilization and lipid storage, GPD1 is frequently examined in models of metabolic stress, oxidative balance, and energy remodeling.
GPD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.