GP49 Antibody (H1.1): sc-53584

GP49 Antibody (H1.1) is a hamster monoclonal IgG antibody that detects GP49 protein of mouse origin by flow cytometry (FCM). GP49 (H1.1) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. GP49 protein is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, primarily expressed on myeloid cells such as mast cells, natural killer (NK) cells, and macrophages, which play crucial roles in both innate and adaptive immunity. GP49 exists in two major isoforms, GP49A and GP49B, encoded by distinct genes that exhibit approximately 95% homology. GP49B serves as an inhibitory isoform, characterized by two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its C-terminal region, which are essential for dampening immune responses. GP49A lacks these inhibitory motifs and has a shorter cytoplasmic domain, allowing potential homodimer formation that can trigger calcium mobilization, eicosanoid production, and cytokine gene transcription, thereby influencing immune signaling pathways. GP49B is homologous to the human Fc receptor for IgA, HM18, which underscores GP49′s importance in immune regulation and potential therapeutic applications.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GP49 Antibody (H1.1) References:

1. gp49: an Ig-like receptor with inhibitory properties on mast cells and natural killer cells.  |  Wagtmann, N. 1999. Curr Top Microbiol Immunol. 244: 107-13. PMID: 10453653
2. Role of exogenous inositol and phosphatidylinositol in glycosylphosphatidylinositol anchor synthesis of GP49 by Giardia lamblia.  |  Subramanian, AB., et al. 2000. Biochim Biophys Acta. 1483: 69-80. PMID: 10601696
3. The gp49A gene has extensive sequence conservation with the gp49B gene and provides gp49A protein, a unique member of a large family of activating and inhibitory receptors of the immunoglobulin superfamily.  |  McCormick, MJ., et al. 1999. Immunogenetics. 50: 286-94. PMID: 10630292
4. Inducible expression of the gp49B inhibitory receptor on NK cells.  |  Wang, LL., et al. 2000. J Immunol. 164: 5215-20. PMID: 10799881
5. Stimulatory function of gp49A, a murine Ig-like receptor, in rat basophilic leukemia cells.  |  Lee, KH., et al. 2000. J Immunol. 165: 4970-7. PMID: 11046024
6. Molecular identification of a novel family of human Ig superfamily members that possess immunoreceptor tyrosine-based inhibition motifs and homology to the mouse gp49B1 inhibitory receptor.  |  Arm, JP., et al. 1997. J Immunol. 159: 2342-9. PMID: 9278324

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GP49 Antibody (H1.1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GP49 Antibody (H1.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GP49 Antibody (H1.1) Specifications

Alternate Product Name: GP49

Product Clone Name: H1.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: GP49

Epitope Species: mouse

Product Reactivity/Detection: GP49A and GP49B

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: FCM

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GP49 Antibody (H1.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin