GnRH I Double Nickase Plasmid (h): sc-401425-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GnRH I Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GnRH I Double Nickase Plasmid (h) and GnRH I Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GNRH1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GnRH I Antibody (HU11B): sc-32292
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GnRH I Double Nickase Plasmid (h) | sc-401425-NIC | 20 µg | $410.00 | |||
GnRH I Double Nickase Plasmid (h2) | sc-401425-NIC-2 | 20 µg | $410.00 |
Human GNRH1 encodes gonadotropin-releasing hormone I (GnRH I), a hypothalamic decapeptide neurohormone that initiates reproductive endocrine signaling by stimulating pituitary gonadotrophs to secrete luteinizing hormone and follicle-stimulating hormone. GnRH I activity integrates neuroendocrine cues that govern puberty onset, fertility, and cyclic reproductive physiology, linking neuronal secretion dynamics to downstream steroidogenesis. Dysregulation of GNRH1 expression or GnRH pathway signaling is associated with disorders of the hypothalamic–pituitary–gonadal axis, including congenital hypogonadotropic hypogonadism and pubertal timing abnormalities, and is frequently interrogated in studies of reproductive endocrinology and neuroendocrine development. Because GnRH signaling influences transcriptional programs in pituitary and gonadal tissues, GNRH1 is also relevant for modeling endocrine feedback and hormone-responsive gene networks.
GnRH I Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GNRH1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GNRH1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GNRH1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GNRH1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.