GMD Double Nickase Plasmid (h): sc-410794-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GMD Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GMD Double Nickase Plasmid (h) and GMD Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GMDS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GMD Antibody (C-7): sc-515226
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GMD Double Nickase Plasmid (h) | sc-410794-NIC | 20 µg | $410.00 |
Human GMDS encodes GDP-mannose 4,6-dehydratase (GMD), a cytosolic enzyme that catalyzes the first committed step in de novo GDP‑L‑fucose biosynthesis from GDP‑mannose. This pathway supplies GDP‑fucose for fucosyltransferase reactions that generate fucosylated glycans on glycoproteins and glycolipids, shaping cell-surface receptor signaling, leukocyte adhesion, and trafficking through modulation of glycan–lectin interactions. GMDS activity influences Golgi-mediated glycosylation programs, including selectin ligand formation and broader glycoprotein maturation that can affect proliferation, immune recognition, and stress responses. Altered fucosylation linked to GMDS dysregulation has been associated with disease-relevant phenotypes in cancer biology and immunology, making GMDS a useful node for studying glycan-dependent regulation of cellular communication.
GMD Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GMDS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GMDS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GMDS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GMDS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.