Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GM2/GD2 Synthase Double Nickase Plasmid (h): sc-403592-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GM2/GD2 Synthase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GM2/GD2 Synthase Double Nickase Plasmid (h) and GM2/GD2 Synthase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting B4GALNT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GM2/GD2 Synthase Antibody (C-5): sc-376505
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GM2/GD2 Synthase Double Nickase Plasmid (h) | sc-403592-NIC | 20 µg | $410.00 | |||
GM2/GD2 Synthase Double Nickase Plasmid (h2) | sc-403592-NIC-2 | 20 µg | $410.00 |
B4GALNT1 encodes GM2/GD2 synthase, a Golgi-resident β1,4-N-acetylgalactosaminyltransferase that catalyzes conversion of lactosylceramide-derived gangliosides to GM2 and GD2, shaping the composition of glycosphingolipids on the plasma membrane. By regulating ganglioside biosynthesis, this enzyme influences membrane microdomain organization, receptor signaling, and cell–cell interactions, linking glycosylation state to pathways controlling adhesion and signal transduction. Altered ganglioside profiles and B4GALNT1 activity have been associated with changes in neural development and neurodegenerative phenotypes, and are frequently examined in contexts where glycosphingolipid remodeling affects cellular identity and stress responses. As a nodal enzyme in glycolipid metabolism, B4GALNT1 is a useful target for dissecting how glycan structure modulates protein trafficking, immune recognition, and surface antigen presentation.
GM2/GD2 Synthase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the B4GALNT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within B4GALNT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt B4GALNT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of B4GALNT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.