GlyR α1 Double Nickase Plasmid (h): sc-404734-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GlyR α1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GlyR α1 Double Nickase Plasmid (h) and GlyR α1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLRA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GlyR α1 Antibody (2E7): sc-293498
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GlyR α1 Double Nickase Plasmid (h) | sc-404734-NIC | 20 µg | $410.00 | |||
GlyR α1 Double Nickase Plasmid (h2) | sc-404734-NIC-2 | 20 µg | $410.00 |
GLRA1 encodes the glycine receptor alpha 1 (GlyR α1) subunit, a ligand-gated chloride channel that mediates fast inhibitory neurotransmission in the spinal cord, brainstem, and other CNS regions. Upon glycine binding, GlyR α1 contributes to chloride influx and membrane hyperpolarization, shaping synaptic inhibition and motor circuit excitability. This receptor participates in neurotransmitter-gated ion channel signaling and inhibitory synapse function, with downstream effects on neuronal firing thresholds and network synchronization. Genetic variation or dysfunction in GLRA1 is associated with altered inhibitory tone and has been linked to startle/hyperekplexia phenotypes and related neurophysiological abnormalities relevant to models of motor control and sensory processing.
GlyR α1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLRA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLRA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLRA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLRA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.