Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
glycogen synthase 2 Double Nickase Plasmid (h): sc-402704-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- glycogen synthase 2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- glycogen synthase 2 Double Nickase Plasmid (h) and glycogen synthase 2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GYS2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: glycogen synthase 2 Antibody (G-8): sc-390391
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
glycogen synthase 2 Double Nickase Plasmid (h) | sc-402704-NIC | 20 µg | $410.00 | |||
glycogen synthase 2 Double Nickase Plasmid (h2) | sc-402704-NIC-2 | 20 µg | $410.00 |
GYS2 encodes human glycogen synthase 2, the predominant isoform responsible for elongating glycogen chains in hepatocytes by transferring glucose from UDP-glucose to existing glycogen primers. Its activity integrates insulin and nutrient signaling through phosphorylation-dependent regulation, coordinating hepatic glycogen storage with systemic glucose availability. GYS2 functions within carbohydrate metabolism pathways that balance glycogenesis and glycogenolysis and is tightly coupled to glucose homeostasis during feeding and fasting cycles. Dysregulation of GYS2-dependent glycogen synthesis is relevant to metabolic phenotypes involving abnormal hepatic glycogen accumulation or depletion and is frequently examined in the context of insulin resistance and broader metabolic disease mechanisms.
glycogen synthase 2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GYS2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GYS2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GYS2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GYS2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.