Molecular structure of Gly-Phe-AFC
Gly-Phe-AFC
Alternate Names:
GF-AFC; Gly-Phe-7-Amino-4-Trifluoromethylcoumarin
Molecular Weight:
433.38
Molecular Formula:
C21H18F3N3O4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Gly-Phe-AFC, a cell-permeant fluorogenic peptide substrate, has been developed for the quantification of viable cells in cytotoxic assays. Upon entering intact cells, this substrate undergoes cleavage, leading to the production of a fluorescent signal that is directly proportional to the number of living cells. Serving as a reliable marker of live-cell protease activity, it loses functionality when membrane integrity is compromised, preventing leakage into the surrounding culture medium. Additionally, Gly-Phe-AFC serves as a substrate for measuring the activity of Dipeptidyl peptidase 1 (DPP1), an intracellular lysosomal cysteinyl protease from the papain family involved in the degradation of proteins.
Gly-Phe-AFC References
- A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers. | Niles, AL., et al. 2007. Anal Biochem. 366: 197-206. PMID: 17512890
- Development and validation of a simple cell-based fluorescence assay for dipeptidyl peptidase 1 (DPP1) activity. | Thong, B., et al. 2011. J Biomol Screen. 16: 36-43. PMID: 21088147
- New approaches for dissecting protease functions to improve probe development and drug discovery. | Deu, E., et al. 2012. Nat Struct Mol Biol. 19: 9-16. PMID: 22218294
- Cell viability assessment: toward content-rich platforms. | Ramirez, CN., et al. 2010. Expert Opin Drug Discov. 5: 223-33. PMID: 22823019
- The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells. | Rao, S., et al. 2015. Biomed Res Int. 2015: 458624. PMID: 26539498
- Serum based fluorescent assay for evaluating dipeptidyl peptidase I activity in collagen induced arthritis rat model. | Liu, X., et al. 2017. Mol Cell Probes. 32: 5-12. PMID: 27771442
- Inhibitory effect of Triperygium wilfordii polyglucoside on dipeptidyl peptidase I in vivo and in vitro. | Wang, J., et al. 2017. Biomed Pharmacother. 96: 466-470. PMID: 29031206
- Liposomes encapsulated dimethyl curcumin regulates dipeptidyl peptidase I activity, gelatinase release and cell cycle of spleen lymphocytes in-vivo to attenuate collagen induced arthritis in rats. | Sun, Z., et al. 2018. Int Immunopharmacol. 65: 511-521. PMID: 30408628
- Identification and Optimization of Novel Cathepsin C Inhibitors Derived from EGFR Inhibitors. | Hou, W., et al. 2019. J Med Chem. 62: 5901-5919. PMID: 31145622
- Multiplexing fluorogenic esterase-based viability assay with luciferase assays. | Ohgane, K., et al. 2019. MethodsX. 6: 2013-2020. PMID: 31667098
- Human breast milk oligosaccharides attenuate necrotizing enterocolitis in rats by suppressing mast cell accumulation, DPPI activity and TLR4 expression in ileum tissue, and regulating mitochondrial damage of Caco-2 cells. | He-Yang, J., et al. 2020. Int Immunopharmacol. 88: 106881. PMID: 32795899
- Causative role of mast cell and mast cell-regulatory function of disialyllacto-N-tetraose in necrotizing enterocolitis. | Zhang, W., et al. 2021. Int Immunopharmacol. 96: 107597. PMID: 33812262
- Disrupted homeostasis of synovial hyaluronic acid and its associations with synovial mast cell proteases of rheumatoid arthritis patients and collagen-induced arthritis rats. | Guo, Y., et al. 2021. Immunol Res. 69: 584-593. PMID: 34482531
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gly-Phe-AFC, 10 mg | sc-506265 | 10 mg | $119.00 | |||
Gly-Phe-AFC, 50 mg | sc-506265A | 50 mg | $312.00 |