Glutathione Peroxidase 7/GPX7 Double Nickase Plasmid (h): sc-404694-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glutathione Peroxidase 7/GPX7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Glutathione Peroxidase 7/GPX7 Double Nickase Plasmid (h) and Glutathione Peroxidase 7/GPX7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPX7. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glutathione Peroxidase 7/GPX7 Double Nickase Plasmid (h) | sc-404694-NIC | 20 µg | $410.00 | |||
Glutathione Peroxidase 7/GPX7 Double Nickase Plasmid (h2) | sc-404694-NIC-2 | 20 µg | $410.00 |
GPX7 encodes glutathione peroxidase 7, an endoplasmic reticulum–localized thiol peroxidase that limits hydrogen peroxide and lipid hydroperoxide accumulation and helps maintain protein folding homeostasis. By supporting redox buffering and ER protein quality control, GPX7 interfaces with oxidative stress responses and unfolded protein response signaling, influencing secretion, proteostasis, and cell survival under stress. Altered GPX7 activity has been linked to dysregulated redox signaling, ER stress sensitivity, and changes in proliferation and apoptosis programs observed across multiple disease-relevant contexts, including inflammation-associated tissue damage and tumor biology. Consequently, GPX7 is frequently studied in models of oxidative injury, proteostasis imbalance, and redox-dependent signaling networks.
Glutathione Peroxidase 7/GPX7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPX7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPX7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPX7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPX7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.