Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Glutathione Peroxidase 1/GPX1 Double Nickase Plasmid (m): sc-420662-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glutathione Peroxidase 1/GPX1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Glutathione Peroxidase 1/GPX1 Double Nickase Plasmid (m) and Glutathione Peroxidase 1/GPX1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Gpx1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glutathione Peroxidase 1/GPX1 Double Nickase Plasmid (m) | sc-420662-NIC | 20 µg | $410.00 |
Mouse Gpx1 encodes glutathione peroxidase 1 (GPX1), a selenium-dependent antioxidant enzyme that reduces hydrogen peroxide and lipid hydroperoxides using glutathione, thereby limiting oxidative damage to proteins, lipids, and DNA. GPX1 activity supports cellular redox homeostasis and intersects with glutathione metabolism, peroxide detoxification, and stress-responsive signaling pathways that influence mitochondrial function and inflammatory responses. Altered GPX1 expression or activity has been linked to oxidative stress phenotypes relevant to metabolic dysfunction, neurodegeneration, and cardiovascular pathology, where dysregulated ROS handling contributes to cellular injury. As a widely expressed cytosolic/peroxisomal enzyme, GPX1 is frequently studied as a node connecting redox balance to gene regulation and adaptive stress responses.
Glutathione Peroxidase 1/GPX1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Gpx1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Gpx1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Gpx1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Gpx1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.