Date published: 2025-9-10
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Glucose Transporter Glut4 Antibody (IF8) - Western Blotting - Image 13605
葡萄糖转运蛋白 Glut4 抗体 (IF8): sc-53566.小鼠骨骼肌组织提取物中 Glut4 表达的 Western 印迹分析.
葡萄糖转运蛋白 Glut4 抗体 (IF8): sc-53566.小鼠骨骼肌组织提取物中 Glut4 表达的 Western 印迹分析.

Glucose Transporter Glut4 抗体 (IF8): sc-53566

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说明书
  • Glucose Transporter Glut4 抗体 IF8 是小鼠单克隆 IgG1 κ，Glucose Transporter Glut4抗体, 在151篇文献中引用，规格为200 µg/ml
  • 针对部分纯化的囊泡，这些囊泡含有来自rat脂肪细胞的Glut4
  • Glucose Transporter Glut4 抗体 (IF8) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Glut4
  • 抗Glucose Transporter Glut4抗体（IF8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Glucose Transporter Glut4 (IF8): sc-53566的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Glucose Transporter Glut4 抗体 (IF8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Glucose Transporter Glut4 抗体 (IF8) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Glut抗体，包括Glut1, Glut10, Glut2, Glut3, Glut4, Glut5和Glut6

Glut4抗体（IF8）是一种IgG1κ小鼠单克隆Glut4抗体（也称为葡萄糖转运蛋白4型抗体、GLUT-4抗体或SLC2A4抗体），可通过WB、IP、IF和IHC（P）检测小鼠、大鼠和人的Glut4蛋白。Glut4抗体（IF8）既有非偶联抗Glut4抗体形式，也有多种偶联形式的抗Glut4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。葡萄糖是哺乳动物细胞代谢的基础。其穿过细胞膜的过程是由一系列被称为葡萄糖转运蛋白或Gluts的转运蛋白介导的。在脂肪和肌肉组织中，胰岛素刺激葡萄糖摄取迅速而显著增加，这主要是由于胰岛素可诱导的葡萄糖转运蛋白Glut4的重新分布。胰岛素反应后，Glut4迅速从细胞内储存位点转移到质膜，在那里与葡萄糖结合。相比之下，普遍表达的葡萄糖转运蛋白Glut1则持续靶向质膜，对胰岛素的反应表现出较少的易位。Glut1和Glut4是十二通道跨膜蛋白（12TM），其羧基末端可能决定它们的细胞定位。异常Glut4的表达已被认为会导致肥胖和糖尿病等疾病。Glut4基因敲除小鼠表明，虽然维持正常葡萄糖水平不需要功能性Glut4蛋白，但它对于持续生长、正常细胞葡萄糖、脂肪代谢和延长寿命是必要的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Glucose Transporter Glut4 抗体 (IF8) 参考文献:

  1. 3T3-L1 脂肪细胞中 GLUT-4 靶向的分子调控。  |  Marsh, BJ., et al. 1995. J Cell Biol. 130: 1081-91. PMID: 7544796
  2. 培养的大鼠脂肪细胞中葡萄糖转运体的调控：胰岛素和地塞米松通过启动子激活对 GLUT4 基因表达的协同作用。  |  Hajduch, E., et al. 1995. Endocrinology. 136: 4782-9. PMID: 7588207
  3. 缺乏 GLUT4 的小鼠会出现心脏和脂肪组织异常，但不会患糖尿病。  |  Katz, EB., et al. 1995. Nature. 377: 151-5. PMID: 7675081
  4. 葡萄糖转运体 GLUT4 在 L6 肌母细胞中的胰岛素敏感靶向是由其 COOH 端细胞质尾部决定的。  |  Haney, PM., et al. 1995. J Cell Biol. 129: 641-58. PMID: 7730401
  5. 洞穴素在脂肪细胞中的表达和分区：与 GLUT4 的协调调控和结构分离。  |  Kandror, KV., et al. 1995. J Cell Biol. 129: 999-1006. PMID: 7744970
  6. 下丘脑 GLUT 4 的表达：葡萄糖和胰岛素感应机制？  |  Livingstone, C., et al. 1995. Mol Cell Endocrinol. 107: 67-70. PMID: 7796936
  7. 有证据表明，促进性葡萄糖转运体可能折叠成β-桶。  |  Fischbarg, J., et al. 1993. Proc Natl Acad Sci U S A. 90: 11658-62. PMID: 8265604

订购信息

产品名称产品编号规格价格数量收藏夹

Glucose Transporter Glut4 抗体 (IF8)

sc-53566
200 µg/ml
$316.00

Glucose Transporter Glut4 (IF8): m-IgG Fc BP-HRP 套装

sc-526827
200 µg Ab; 10 µg BP
$354.00

Glucose Transporter Glut4 (IF8): m-IgG1 BP-HRP 套装

sc-532200
200 µg Ab; 20 µg BP
$354.00

Glucose Transporter Glut4 抗体 (IF8) AC

sc-53566 AC
500 µg/ml, 25% agarose
$416.00

Glucose Transporter Glut4 抗体 (IF8) HRP

sc-53566 HRP
200 µg/ml
$316.00

Glucose Transporter Glut4 抗体 (IF8) FITC

sc-53566 FITC
200 µg/ml
$330.00

Glucose Transporter Glut4 抗体 (IF8) PE

sc-53566 PE
200 µg/ml
$343.00

Glucose Transporter Glut4 抗体 (IF8) Alexa Fluor® 488

sc-53566 AF488
200 µg/ml
$357.00

Glucose Transporter Glut4 抗体 (IF8) Alexa Fluor® 546

sc-53566 AF546
200 µg/ml
$357.00

Glucose Transporter Glut4 抗体 (IF8) Alexa Fluor® 594

sc-53566 AF594
200 µg/ml
$357.00

Glucose Transporter Glut4 抗体 (IF8) Alexa Fluor® 647

sc-53566 AF647
200 µg/ml
$357.00

Glucose Transporter Glut4 抗体 (IF8) Alexa Fluor® 680

sc-53566 AF680
200 µg/ml
$357.00

Glucose Transporter Glut4 抗体 (IF8) Alexa Fluor® 790

sc-53566 AF790
200 µg/ml
$357.00

I am going to try sc-53566 FITC for IF in rat L6 myotubes. Do you have a tested protocol for fixing and permeabilization for this GLUT4 antibody?

Asked by: STed
Thank you for your question. Here is our protocol : https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2023-12-29

what is an isotype control for the a-GLUT4 antibody I can use?

Asked by: Elena K sanofi
Thank you for your questions. We recommend using a mouse igG1 isotype control. Here is a link to the controls we offer: https://www.scbt.com/browse/Control-IgGs-and-IgG-Conjugates/_/N-1j3ajah?Nr=AND%28OR%28product.catalogId%3AscbtCatalog%29%2Cproduct.discontinuedFlag%3A0%2Cproduct.siteId%3AscbtSite%2Cproduct.webDisplayFlag%3A1%2Cproduct.suppressDisplayFlag%3A0%29&autoSuggest=true&dataType=json
Answered by: BlakeJ
Date published: 2022-11-14

Buenas tardes, me gustaría saber si el anticuerpo sc-53566 está dirigido contra la región extracelular de GLUT4. Si no es así, ¿tienen algún aticuerpo que esté dirigido contra esta región? Muchas Gracias

Asked by: Alba96
Gracias por su pregunta. No se ha realizado un mapeo de epítopos para el anticuerpo Glut4 transportador de glucosa (IF8): sc-53566 hasta el momento, por lo que no podemos decir qué epítopo reconoce. Este es el único anticuerpo específico contra este objetivo que ofrecemos. Thank you for your question. No epitope mapping has been performed for Glucose Transporter Glut4 Antibody (IF8): sc-53566 as of yet, so we cannot say what epitope it recognises. This is the only antibody specific against this target that we offer.
Answered by: Tech Support Europe
Date published: 2022-05-30

What dilution do you recommend for western blot?

Asked by: AJ99
Thank you for your question. We recommend a starting dilution of 1:100 - 1:1000. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Blake J
Date published: 2021-09-22

Is this antibody targeting the N-terminal extracellular domain of Glut4 of human origin?

Asked by: ClariSB
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2020-04-22

I I noticed that you have a free trial of this antibody Glut4 Antibody (IF8): sc-53566, can you give us a try?

Asked by: SUNNY2
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-05-14

Hello, I want to use this antibody for immunostaining GLUT-4 in nonpermeabilized cells to selectively stain the surface GLUT-4. Should this antibody work for my experiment? I am curious whether this antibody recognizes extracellular epitope of GLUT-4.

Asked by: Ritwik
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. We have not tested this antibody in non-permeablized cells. You may want to check some of the product citations using this antibody to see if others have previously published in the same application.
Answered by: Technical Support
Date published: 2025-07-28

hello I have bought glu t4 if8: sc-53566 for counting glu t4 on adipose cell surface. what are the emission and excision wave length for if-8? your sincerely hasannejad

Asked by: hasannejad
Thank you for posting your question. The relevant excitation and emission wavelengths depend on which fluorochrome conjugate you are inquiring about. We have many different options available. Please contact Technical Service in Europe for more detailed information. They can be reached via chat or phone at Toll Free: +00800 4573 8000.
Answered by: Tech Help
Date published: 2019-06-18
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Rated 5 out of 5 by from 功能出色此抗體靈敏度高特異性強，背景雜訊低操作，且簡便適用多種樣本實驗，結果穩定信賴度高反應效果佳!!!!!
Date published: 2025-07-18
Rated 5 out of 5 by from GOODthis antibody works well in skeletal muscle of mice
Date published: 2023-05-29
Rated 5 out of 5 by from GLUT4 AntibodyGLUT4 Antibody is a highly specific antibody for western blot!
Date published: 2023-03-24
Rated 1 out of 5 by from Not working in 1:20 concentration in 5%milk/TBS-TTested on mice brain cortex, fresh membranes, 1:200 antibody in 5%milk in TBS-T - overnight 4*C and then 1:4000 secondary anti-mouse IgG Fc in 5%milk in TBS-T for 1h.
Date published: 2023-03-17
Rated 4 out of 5 by from This antibody works well for both IF and WesternsI've used this antibody to assess GLUT4 expression both on cell membranes, by IF, and by Western blot. A 1:200 dilution worked well for both methods. I'd recommend this antibody.
Date published: 2022-06-10
Rated 5 out of 5 by from NiceThe antibody works well for WB on rat cardiac muscle cell.
Date published: 2022-03-03
Rated 5 out of 5 by from Ottimo funzionamento!L'anticorpo testato si è dimostrato funzionante. E' assolutamente da raccomandare!
Date published: 2020-11-03
Rated 5 out of 5 by from Great for GLUT analysis in skeletal muscleExcellent antibody. The best GLUT4 antibody I have tested in WB
Date published: 2018-07-24
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Glucose Transporter Glut4 抗体 (IF8) is rated 4.0 out of 5 by 31.
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