GluR-δ2 Double Nickase Plasmid (h): sc-403400-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GluR-δ2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GluR-δ2 Double Nickase Plasmid (h) and GluR-δ2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRID2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GluR-δ2 Antibody (D-9): sc-393437
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GluR-δ2 Double Nickase Plasmid (h) | sc-403400-NIC | 20 µg | $410.00 | |||
GluR-δ2 Double Nickase Plasmid (h2) | sc-403400-NIC-2 | 20 µg | $410.00 |
GRID2 encodes glutamate receptor delta-2 (GluR-δ2), an ionotropic glutamate receptor family member that is highly enriched in cerebellar Purkinje neurons and functions primarily as a synaptic organizer rather than a canonical ligand-gated channel. GluR-δ2 participates in trans-synaptic signaling complexes that regulate parallel fiber–Purkinje cell synapse formation, synaptic maintenance, and activity-dependent plasticity, influencing intracellular pathways linked to spine remodeling and long-term depression. Altered GRID2 function or expression is associated with disrupted cerebellar circuitry and has been implicated in neurodevelopmental and neurodegenerative phenotypes characterized by impaired motor coordination and ataxia. As a result, GRID2 is frequently studied in mechanisms of synaptogenesis, excitatory synapse specification, and cerebellar network physiology.
GluR-δ2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRID2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRID2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRID2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRID2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.