Glucose Transporter Glut5 CRISPR Activation Plasmid (h): sc-401907-ACT

SLC2A5 encodes glucose transporter Glut5, a facilitative membrane carrier with high specificity for fructose that supports hexose uptake across the plasma membrane and contributes to cellular energy balance and carbon flux. Glut5-mediated transport influences glycolytic entry points and interfaces with nutrient-sensing programs, linking extracellular sugar availability to metabolic reprogramming. Altered SLC2A5 expression has been studied in contexts of intestinal fructose absorption, metabolic stress, and tumor cell nutrient utilization, where changes in fructose transport can impact proliferation and oxidative metabolism. As a cell-surface transporter, Glut5 also serves as a tractable node for investigating membrane trafficking, substrate competition among SLC2 family members, and metabolic pathway dependencies.

Glucose Transporter Glut5 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous SLC2A5 expression without altering the underlying DNA sequence.

Glucose Transporter Glut5 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the SLC2A5 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.

Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the SLC2A5 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Glucose Transporter Glut5 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native SLC2A5 locus and enabling the study of Glucose Transporter Glut5-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Glucose Transporter Glut5 pathway restoration in tumor cells with silenced or reduced SLC2A5 expression.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.