Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Glucose Transporter Glut10 CRISPR/Cas9 KO Plasmid (m): sc-431260
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glucose Transporter Glut10 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Glucose Transporter Glut10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Glucose Transporter Glut10 Antibody (H-10): sc-398495
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucose Transporter Glut10 CRISPR/Cas9 KO Plasmid (m) | sc-431260 | 20 µg | $397.00 |
Overview
Slc2a10 encodes glucose transporter GLUT10, a facilitative hexose transporter that contributes to cellular carbohydrate handling and redox homeostasis in mouse tissues. GLUT10 has been linked to intracellular transport of glucose or dehydroascorbate, connecting sugar flux with antioxidant pathways that influence mitochondrial function and extracellular matrix maintenance. Through these roles, Slc2a10 is relevant to studies of metabolic adaptation, oxidative stress responses, and vascular connective tissue biology. Genetic disruption of Slc2a10 is used to probe mechanisms underlying arterial tortuosity syndrome–like phenotypes, altered collagen/elastin organization, and stress-sensitive metabolic remodeling in experimental models.
Glucose Transporter Glut10 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc2a10 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc2a10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc2a10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glucose Transporter Glut10 protein expression.
This CRISPR knockout system enables efficient generation of Slc2a10-deficient cell models for investigation of Glucose Transporter Glut10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc2a10 exon(s) critical for Glucose Transporter Glut10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc2a10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Glucose Transporter Glut10 CRISPR/Cas9 KO Plasmid (m) and Glucose Transporter Glut10 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc2a10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Glucose Transporter Glut10 HDR Plasmid (m) and Glucose Transporter Glut10 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc2a10 homology arms to support homology-directed repair at defined Slc2a10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.