GLTSCR1L Double Nickase Plasmid (h): sc-407155-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GLTSCR1L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GLTSCR1L Double Nickase Plasmid (h) and GLTSCR1L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLTSCR1L. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLTSCR1L Double Nickase Plasmid (h) | sc-407155-NIC | 20 µg | $410.00 | |||
GLTSCR1L Double Nickase Plasmid (h2) | sc-407155-NIC-2 | 20 µg | $410.00 |
GLTSCR1L (glioma tumor suppressor candidate region gene 1-like) encodes a nuclear protein implicated in the regulation of chromatin-associated processes that influence transcriptional programs and genome organization. Although its molecular partners are still being defined, available evidence supports roles in pathways controlling epigenetic regulation, RNA processing, and cell-state maintenance through modulation of nuclear protein complexes. Perturbation of GLTSCR1L expression has been observed in cancer-associated datasets, consistent with potential involvement in cellular proliferation and differentiation control. These features make GLTSCR1L a useful target for dissecting nuclear regulatory mechanisms linked to oncogenic signaling and lineage specification in human cells.
GLTSCR1L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLTSCR1L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLTSCR1L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLTSCR1L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLTSCR1L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.