GlcAT-I Antibody (D-7): sc-390475

GlcAT-I Antibody (D-7) is a mouse monoclonal IgM antibody that detects GlcAT-I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GlcAT-I antibody (D-7) is available as the non-conjugated form. GlcAT-I, also known as beta-1,3-glucuronyltransferase 3 (B3GAT3), is a 335 amino acid single-pass type II membrane protein that belongs to the glycosyltransferase 43 family. GlcAT-I plays a crucial role in proteoglycan biosynthesis by catalyzing the formation of the glycosaminoglycan-protein linkage through a glucuronyl transfer reaction, utilizing manganese as a cofactor. GlcAT-I shows strict specificity for the Gal-beta-1,3-Gal-beta-1,4-Xyl linkage, which is essential for the structural integrity and function of proteoglycans in the extracellular matrix. GlcAT-I exists as a disulfide-linked homodimer and is ubiquitously expressed, with localization primarily in the Golgi apparatus membrane, where N-glycosylation occurs to influence activity and stability.

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GlcAT-I Antibody (D-7) References:

1. Characterization of recombinant human glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans.  |  Tone, Y., et al. 1999. FEBS Lett. 459: 415-20. PMID: 10526176
2. Structure/function of the human Ga1beta1,3-glucuronosyltransferase. Dimerization and functional activity are mediated by two crucial cysteine residues.  |  Ouzzine, M., et al. 2000. J Biol Chem. 275: 28254-60. PMID: 10842173
3. Heparan/chondroitin sulfate biosynthesis. Structure and mechanism of human glucuronyltransferase I.  |  Pedersen, LC., et al. 2000. J Biol Chem. 275: 34580-5. PMID: 10946001
4. The functional glycosyltransferase signature sequence of the human beta 1,3-glucuronosyltransferase is a XDD motif.  |  Gulberti, S., et al. 2003. J Biol Chem. 278: 32219-26. PMID: 12794088
5. Phosphorylation and sulfation of oligosaccharide substrates critically influence the activity of human beta1,4-galactosyltransferase 7 (GalT-I) and beta1,3-glucuronosyltransferase I (GlcAT-I) involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans.  |  Gulberti, S., et al. 2005. J Biol Chem. 280: 1417-25. PMID: 15522873
6. Stimulation of proteoglycan synthesis by glucuronosyltransferase-I gene delivery: a strategy to promote cartilage repair.  |  Venkatesan, N., et al. 2004. Proc Natl Acad Sci U S A. 101: 18087-92. PMID: 15601778
7. Molecular basis for acceptor substrate specificity of the human beta1,3-glucuronosyltransferases GlcAT-I and GlcAT-P involved in glycosaminoglycan and HNK-1 carbohydrate epitope biosynthesis, respectively.  |  Fondeur-Gelinotte, M., et al. 2007. Glycobiology. 17: 857-67. PMID: 17567734
8. 2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis.  |  Tone, Y., et al. 2008. J Biol Chem. 283: 16801-7. PMID: 18400750
9. Molecular cloning and expression of glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans.  |  Kitagawa, H., et al. 1998. J Biol Chem. 273: 6615-8. PMID: 9506957

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GlcAT-I Antibody (D-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-GlcAT-I Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GlcAT-I Antibody (D-7) Specifications

Alternate Product Name: GlcAT-I

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 323-334

Epitope Target: GlcAT-I

Epitope Species: human

Product Reactivity/Detection: GlcAT-I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for GlcAT-I Antibody (D-7): P: Blocking Peptide