Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gl Syn Double Nickase Plasmid (h): sc-401090-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gl Syn Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Gl Syn Double Nickase Plasmid (h) and Gl Syn Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLUL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gl Syn Antibody (E-4): sc-74430
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gl Syn Double Nickase Plasmid (h) | sc-401090-NIC | 20 µg | $410.00 | |||
Gl Syn Double Nickase Plasmid (h2) | sc-401090-NIC-2 | 20 µg | $410.00 |
GLUL encodes glutamine synthetase (Gl Syn), a key cytosolic enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine, thereby supporting nitrogen assimilation and amino acid homeostasis. By controlling intracellular glutamine pools, GLUL influences central carbon metabolism, anaplerosis, and biosynthetic pathways linked to nucleotide and protein synthesis. GLUL activity is integrated with glutamate–glutamine cycling, redox balance, and cellular responses to nutrient availability. Dysregulated GLUL expression or function has been associated with altered metabolic states observed in cancer biology, neurological physiology, and liver-related metabolic processes, making it relevant for mechanistic studies of metabolic reprogramming.
Gl Syn Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLUL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLUL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLUL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLUL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.