Gigaxonin Antibody (F-3): sc-376173

Gigaxonin Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects Gigaxonin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Gigaxonin (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gigaxonin, also known as giant axonal neuropathy, GAN1, or KLHL16, plays a critical role in protein degradation regulation, which is essential for maintaining neuronal function and survival. Gigaxonin belongs to the cytoskeletal BTB/kelch repeat family and influences cytoskeletal organization and dynamics, particularly in neurofilament architecture. The amino-terminal BTB domain binds to ubiquitin-activating enzyme E1, while the carboxy-terminal kelch repeat domain interacts with microtubule-associated protein 1B (MAP1B) light chain, marking MAP1B for degradation. This interaction remains crucial because MAP1B overexpression can lead to neuronal cell death, whereas reduced MAP1B levels significantly enhance neuronal survival. Mutations in Gigaxonin cause human giant axonal neuropathy (GAN), an autosomal recessive neurodegenerative disorder characterized by axonal degeneration due to cytoskeletal abnormalities, including intermediate filament accumulation.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Gigaxonin Antibody (F-3) References:

1. Gigaxonin is associated with the Golgi and dimerises via its BTB domain.  |  Cullen, VC., et al. 2004. Neuroreport. 15: 873-6. PMID: 15073534
2. Degradation of the Intermediate Filament Family by Gigaxonin.  |  Bomont, P. 2016. Methods Enzymol. 569: 215-31. PMID: 26778561
3. A review of gigaxonin mutations in giant axonal neuropathy (GAN) and cancer.  |  Kang, JJ., et al. 2016. Hum Genet. 135: 675-84. PMID: 27023907
4. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production.  |  Scrivo, A., et al. 2019. Nat Commun. 10: 780. PMID: 30770803
5. Gigaxonin glycosylation regulates intermediate filament turnover and may impact giant axonal neuropathy etiology or treatment.  |  Chen, PH., et al. 2020. JCI Insight. 5: PMID: 31944090
6. E3 Ubiquitin Ligases in Neurological Diseases: Focus on Gigaxonin and Autophagy.  |  Lescouzères, L. and Bomont, P. 2020. Front Physiol. 11: 1022. PMID: 33192535
7. Gigaxonin is required for intermediate filament transport.  |  Renganathan, B., et al. 2023. FASEB J. 37: e22886. PMID: 37043392
8. Axonal Transport Defect in Gigaxonin Deficiency Rescued by Tubastatin A.  |  Nath, B., et al. 2023. Neurotherapeutics. 20: 1215-1228. PMID: 37268847
9. The CRL3gigaxonin ubiquitin ligase-USP15 pathway governs the destruction of neurofilament proteins.  |  Park, HM., et al. 2023. Proc Natl Acad Sci U S A. 120: e2306395120. PMID: 37903270
10. Gigaxonin, mutated in Giant Axonal Neuropathy, interacts with TDP-43 and other RNA binding proteins.  |  Phillips, CL., et al. 2024. bioRxiv.. PMID: 39282431

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Gigaxonin Antibody (F-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Gigaxonin Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Gigaxonin Antibody (F-3) Specifications

Alternate Product Name: Gigaxonin

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 298-597

Epitope Target: Gigaxonin

Epitope Species: human

Product Reactivity/Detection: Gigaxonin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Gigaxonin Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin