Giantin Antibody (2388C3a): sc-81279

Giantin Antibody (2388C3a) is a mouse monoclonal IgG1 kappa light chain antibody that detects Giantin protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-Giantin antibody (2388C3a) is available as a non-conjugated format and plays a crucial role in the structure and function of the Golgi apparatus. Giantin protein, also known as macrogolgin or Golgi complex-associated protein, is a single-pass type I membrane protein that forms a homodimer and is integral to the organization of the Golgi complex. Giantin (2388C3a) antibody targets a protein that projects significantly into the cytoplasm, facilitating interactions with other Golgi proteins such as GM130 and p115, which are essential for vesicle tethering and docking to the Golgi membrane. This interaction is vital for maintaining the integrity of the Golgi apparatus and ensuring proper protein trafficking within the cell. Giantin monoclonal antibody (2388C3a) recognizes an antigen involved in autoimmune conditions such as Sjögren′s syndrome and chronic rheumatoid arthritis, highlighting its importance not only in cellular function but also in disease pathology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Giantin Antibody (2388C3a) References:

1. Autoantibodies to protein transport and messenger RNA processing pathways: endosomes, lysosomes, Golgi complex, proteasomes, assemblyosomes, exosomes, and GW bodies.  |  Stinton, LM., et al. 2004. Clin Immunol. 110: 30-44. PMID: 14962794
2. Progressive myopathy with circulating autoantibody against giantin in the Golgi apparatus.  |  Sahashi, K., et al. 2004. Neurology. 62: 1891-3. PMID: 15159505
3. The inositol polyphosphate 5-phosphatase Ocrl associates with endosomes that are partially coated with clathrin.  |  Ungewickell, A., et al. 2004. Proc Natl Acad Sci U S A. 101: 13501-6. PMID: 15353600
4. Golgin tethers define subpopulations of COPI vesicles.  |  Malsam, J., et al. 2005. Science. 307: 1095-8. PMID: 15718469
5. A cryptic Rab1-binding site in the p115 tethering protein.  |  Beard, M., et al. 2005. J Biol Chem. 280: 25840-8. PMID: 15878873
6. Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression.  |  Stevenson, NL., et al. 2017. J Cell Sci. 130: 4132-4143. PMID: 29093022
7. Giantin is required for coordinated production of aggrecan, link protein and type XI collagen during chondrogenesis.  |  Katayama, K., et al. 2018. Biochem Biophys Res Commun. 499: 459-465. PMID: 29577904
8. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells.  |  Casey, CA., et al. 2018. Biomolecules. 8: PMID: 30453527
9. The Golgin Protein Giantin Regulates Interconnections Between Golgi Stacks.  |  Satoh, A., et al. 2019. Front Cell Dev Biol. 7: 160. PMID: 31544102
10. Giantin is required for intracellular N-terminal processing of type I procollagen.  |  Stevenson, NL., et al. 2021. J Cell Biol. 220: PMID: 33944912
11. Molecular genetic analyses of a 376-kilodalton Golgi complex membrane protein (giantin).  |  Seelig, HP., et al. 1994. Mol Cell Biol. 14: 2564-76. PMID: 7511208
12. Molecular cloning and sequence analysis of a human 372-kDA protein localized in the Golgi complex.  |  Sohda, M., et al. 1994. Biochem Biophys Res Commun. 205: 1399-408. PMID: 7802676

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Giantin Antibody (2388C3a) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Giantin Antibody (2388C3a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Giantin Antibody (2388C3a) Specifications

Alternate Product Name: GOLGB1

Product Clone Name: 2388C3a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Giantin

Epitope Species: human

Product Reactivity/Detection: Giantin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Giantin Antibody (2388C3a): N/A