Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GHR Double Nickase Plasmid (h): sc-401382-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GHR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GHR Double Nickase Plasmid (h) and GHR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GHR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GHR Antibody (B-10): sc-137185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GHR Double Nickase Plasmid (h) | sc-401382-NIC | 20 µg | $410.00 | |||
GHR Double Nickase Plasmid (h2) | sc-401382-NIC-2 | 20 µg | $410.00 |
GHR encodes the growth hormone receptor, a single-pass transmembrane cytokine receptor that binds pituitary growth hormone to regulate postnatal growth, metabolism, and tissue homeostasis. Ligand-induced receptor dimerization activates JAK2 and downstream STAT5 signaling, with additional coupling to PI3K–AKT and MAPK/ERK pathways that shape proliferation, survival, and transcriptional programs. GHR signaling influences hepatic IGF-1 production and broader endocrine feedback loops, integrating nutrient status with anabolic and lipolytic responses. Dysregulated GHR activity and pathway crosstalk have been studied in contexts including growth disorders, insulin sensitivity phenotypes, and oncogenic signaling networks where JAK/STAT and MAPK outputs contribute to disease-relevant cellular states.
GHR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GHR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GHR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GHR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GHR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.