Ggnbp2 Double Nickase Plasmid (m): sc-432161-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ggnbp2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ggnbp2 Double Nickase Plasmid (m) and Ggnbp2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ggnbp2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ggnbp2 Double Nickase Plasmid (m) | sc-432161-NIC | 20 µg | $410.00 | |||
Ggnbp2 Double Nickase Plasmid (m2) | sc-432161-NIC-2 | 20 µg | $410.00 |
Mouse Ggnbp2 (gametogenetin binding protein 2) encodes a predominantly nuclear protein implicated in transcriptional regulation and chromatin-associated processes, with reported roles in cell-cycle progression and genome stability. Expression has been noted in proliferative and reproductive tissues, supporting investigation of Ggnbp2 in germline development and mitotic control. Altered GGNBP2 activity has been associated in the literature with dysregulated proliferation and oncogenic phenotypes, making it relevant for studies of tumor suppressive networks and differentiation programs. Functional interrogation of Ggnbp2 can clarify how nuclear regulatory factors integrate signaling cues with gene expression and cellular homeostasis.
Ggnbp2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ggnbp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ggnbp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ggnbp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ggnbp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.