GFAP Antibody (F-2): sc-166481
- GFAP Antibody (F-2) is a mouse monoclonal IgG2b κ, cited in 15 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-19 at the N-terminus of GFAP of human origin
- Anti-GFAP Antibody (F-2) is recommended for detection of GFAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-GFAP Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GFAP Antibody (F-2) for WB and IHC(P) applications. These reagents are now offered in bundles with GFAP Antibody (F-2) (see ordering information below).
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SEE ALSO...
GFAP Antibody (F-2) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect glial fibrillary acidic protein (GFAP), an essential intermediate filament protein predominantly located within the cytoskeleton of astroglial cells in the central nervous system. Targeting an epitope between amino acids 2-19 at the N-terminus of human GFAP, anti-GFAP antibody (F-2) effectively recognizes GFAP from mouse, rat, and human species, making GFAP (F-2) antibody suitable for a wide range of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). The precise localization of GFAP within the astrocyte cytoskeleton is crucial for maintaining cellular integrity, facilitating intracellular transport, and enabling astrocytes to respond dynamically to neural injury and stress. By stabilizing the astrocyte structure, GFAP plays a vital role in supporting neuronal function, regulating neurotransmitter uptake, and maintaining the blood-brain barrier. Additionally, GFAP′s interaction with other cytoskeletal proteins and signaling molecules is essential for astrocyte-mediated processes such as cell migration, proliferation, and response to inflammatory stimuli. GFAP monoclonal antibody (F-2) offers high specificity with minimal cross-reactivity, allowing for accurate identification and localization of astrocytes and related glial cells in various tissues. Anti-GFAP antibody (F-2) is particularly valuable for studying astrogliosis, a reactive process marked by increased GFAP expression in response to neurological insults like prion infections, AIDS related dementia, mechanical trauma, and inflammatory demyelinating diseases, thereby providing critical insights into the pathophysiology of numerous central nervous system disorders. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® variants, GFAP (F-2) monoclonal antibody is an indispensable tool for researchers investigating the molecular mechanisms of neural health and disease, facilitating a deeper understanding of astrocyte function and their pivotal role in maintaining the structural and functional integrity of the central nervous system.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GFAP Antibody (F-2) | sc-166481 | 200 µg/ml | $316.00 | |||
GFAP Antibody (F-2): m-IgGκ BP-HRP Bundle | sc-521627 | 200 µg Ab, 40 µg BP | $354.00 | |||
GFAP Antibody (F-2): m-IgG2b BP-HRP Bundle | sc-548942 | 200 µg Ab; 10 µg BP | $354.00 | |||
GFAP Antibody (F-2) AC | sc-166481 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GFAP Antibody (F-2) HRP | sc-166481 HRP | 200 µg/ml | $316.00 | |||
GFAP Antibody (F-2) FITC | sc-166481 FITC | 200 µg/ml | $330.00 | |||
GFAP Antibody (F-2) PE | sc-166481 PE | 200 µg/ml | $343.00 | |||
GFAP Antibody (F-2) Alexa Fluor® 488 | sc-166481 AF488 | 200 µg/ml | $357.00 | |||
GFAP Antibody (F-2) Alexa Fluor® 546 | sc-166481 AF546 | 200 µg/ml | $357.00 | |||
GFAP Antibody (F-2) Alexa Fluor® 594 | sc-166481 AF594 | 200 µg/ml | $357.00 | |||
GFAP Antibody (F-2) Alexa Fluor® 647 | sc-166481 AF647 | 200 µg/ml | $357.00 | |||
GFAP Antibody (F-2) Alexa Fluor® 680 | sc-166481 AF680 | 200 µg/ml | $357.00 | |||
GFAP Antibody (F-2) Alexa Fluor® 790 | sc-166481 AF790 | 200 µg/ml | $357.00 | |||
GFAP (F-2) Neutralizing Peptide | sc-166481 P | 100 µg/0.5 ml | $68.00 |