GENIUS™ Nuclease DMF Filed (CAS 9025-65-4)
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GENIUS™ Nuclease DMF Filed Lyophilized Powder is a solvent-free version of GENIUS™ Nuclease. GENIUS™ Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts. The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. GENIUS™ Nuclease is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples. Unit definition: One unit of GENIUS™ Nuclease DMF Filed is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units. Benzonase is a registered trademark of Merck KGaA.
GENIUS™ Nuclease DMF Filed (CAS 9025-65-4) References
- Disulfide bonds are required for Serratia marcescens nuclease activity. | Ball, TK., et al. 1992. Nucleic Acids Res. 20: 4971-4. PMID: 1329033
- The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli. | Ball, TK., et al. 1987. Gene. 57: 183-92. PMID: 3319779
- 2.1 A structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA. | Miller, MD., et al. 1994. Nat Struct Biol. 1: 461-8. PMID: 7664065
- A new mass spectrometric approach to detect modifications in DNA. | Janning, P., et al. 1994. Rapid Commun Mass Spectrom. 8: 1035-40. PMID: 7696699
- Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis. | Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-7. PMID: 8078761
- Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. | Molloy, MP., et al. 1998. Electrophoresis. 19: 837-44. PMID: 9629924
- Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. | Herbert, BR., et al. 1998. Electrophoresis. 19: 845-51. PMID: 9629925
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GENIUS™ Nuclease DMF Filed, 10 KU | sc-391121A | 10 KU | $95.00 | |||
GENIUS™ Nuclease DMF Filed, 25 KU | sc-391121 | 25 KU | $156.00 | |||
GENIUS™ Nuclease DMF Filed, 50 KU | sc-391121B | 50 KU | $218.00 | |||
GENIUS™ Nuclease DMF Filed, 500 KU | sc-391121C | 500 KU | $952.00 |