Geneticin (G418) Sulfate: sc-29065. Cultured HeLa cells treated with 0μg/ml (A) and 8μg/ml (B) Geneticin (G418) Sulfate, for 48 hours and subsequently stained with Trypan Blue: sc-216028 to check for cell viability.
Geneticin (G418) Sulfate (CAS 108321-42-2)
See product citations (193)
Alternate Names:
Geneticin (G418) Sulfate is also known as Antibiotic G418
Application:
Geneticin (G418) Sulfate is an aminoglycoside antibiotic which is derived from Micromonospora rhodoranga NRRL 5326
CAS Number:
108321-42-2
Molecular Weight:
692.71
Molecular Formula:
C20H40N4O10•2H2SO4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Geneticin (G418) Sulfate, also designated Antibiotic G418, is an aminoglycoside antibiotic found in Micromonospora rhodoranga NRRL 5326. Geneticin (G418) Sulfate may act to cause a bypass of nonsense mutations during translation. Experiments have shown that Geneticin (G418) Sulfate inhibits the incorporation of amino acids into protein and can destroy fibroblasts. Mechanistic studies suggest that Geneticin (G418) Sulfate affects the 80S ribosome and inhibits the elongation cycle. Further studies report that Geneticin (G418) Sulfate is highly active against amoebae, protozoa, tapeworm, and pinworm exposed murine cells.
Geneticin (G418) Sulfate (CAS 108321-42-2) References
- Cellular responses to sodium butyrate exhibit the dominance of one parental phenotype in somatic cell hybrids. | Cox, GS. 2000. Mol Cell Biol Res Commun. 3: 329-37. PMID: 11032754
- The rat neurotensin receptor expressed in Chinese hamster ovary cells mediates the release of inositol phosphates. | Watson, MA., et al. 1992. J Neurochem. 59: 1967-70. PMID: 1328536
- Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation, and preliminary characterization. | Wagman, GH., et al. 1974. Antimicrob Agents Chemother. 6: 144-9. PMID: 15828184
- A mouse model for nonsense mutation bypass therapy shows a dramatic multiday response to geneticin. | Yang, C., et al. 2007. Proc Natl Acad Sci U S A. 104: 15394-9. PMID: 17881586
- Expression of GFP using Pichia pastoris vectors with zeocin or G-418 sulphate as the primary selectable marker. | Papakonstantinou, T., et al. 2009. Yeast. 26: 311-21. PMID: 19399907
- Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector. | LaFace, D., et al. 1988. Virology. 162: 483-6. PMID: 2829430
- Improving the baculovirus expression vector system with vankyrin-enhanced technology. | Steele, KH., et al. 2017. Biotechnol Prog. 33: 1496-1507. PMID: 28649776
- Transfection of lymphoblastoid cells using DNA-loaded reconstituted Sendai virus envelopes: expression of transfected DNA and selection of transfected cells. | Shapiro, IM., et al. 1986. Somat Cell Mol Genet. 12: 351-6. PMID: 3016915
- DNA-mediated transformation in the aquatic filamentous fungus Achlya ambisexualis. | Manavathu, EK., et al. 1988. J Gen Microbiol. 134: 2019-28. PMID: 3246595
- Infection of a murine T-cell line with a retrovirus carrying c-myc decreases the interleukin-2 cell dependence by a nonautocrine mechanism. | Shapiro, IM., et al. 1988. J Leukoc Biol. 44: 559-65. PMID: 3264010
- G-418, an elongation inhibitor of 80 S ribosomes. | Bar-Nun, S., et al. 1983. Biochim Biophys Acta. 741: 123-7. PMID: 6193810
- Selective elimination of fibroblasts from cultures of normal human melanocytes. | Halaban, R. and Alfano, FD. 1984. In Vitro. 20: 447-50. PMID: 6724622
- Evaluation of expression of transferred genes in differentiating myeloid cells: expression of human glucocerebrosidase in murine macrophages. | Freas, DL., et al. 1993. Hum Gene Ther. 4: 283-90. PMID: 7687878
- A reporter gene to analyse the hypermutation of immunoglobulin genes. | Chui, YL., et al. 1995. J Mol Biol. 249: 555-63. PMID: 7783211
- Development of a high-performance liquid chromatographic assay for G418 sulfate (Geneticin). | Bethune, C., et al. 1997. Antimicrob Agents Chemother. 41: 661-4. PMID: 9056010
Inhibitor of:
C14orf21, C19orf61, eRF3a, GPI, NRSF, PHGR1, Ribosomal Protein L22, Ribosome (RPL; 40S; 60S), and Sup35.Activator of:
DDX51, eRF1, ETEA, Histone H3.3B, Imp4, and SMG8.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Geneticin (G418) Sulfate, 1 g | sc-29065 | 1 g | $106.00 | |||
Geneticin (G418) Sulfate, 5 g | sc-29065A | 5 g | $381.00 | |||
Geneticin (G418) Sulfate, 25 g | sc-29065B | 25 g | $459.00 | |||
Geneticin (G418) Sulfate, 100 g | sc-29065C | 100 g | $1500.00 | |||
Geneticin (G418) Sulfate, 500 g | sc-29065D | 500 g | $6125.00 |