Gemcitabine Monophosphate Formate Salt (CAS 116371-67-6)

Gemcitabine Monophosphate Formate Salt is a deoxycytidine analog. Gemcitabine Monophosphate Formate Salt functions by incorporating itself in the DNA of the cell and blocking DNA polymerases. Gemcitabine Monophosphate Formate Salt also inhibits R1 and R2 (ribonucleotide reductase) via irreversibly binding to the enzymes active site. This inhibition stops the production of deoxyribonucleotides and so DNA replication ceases.Gemcitabine Monophosphate Formate Salt functions as a cytidine analog and a nucleoside analog, mimicking cytidine and nucleosides found in nucleic acids.The activation process involves the conversion of gemcitabine monophosphate into gemcitabine by deoxycytidine kinase. Subsequently, gemcitabine is phosphorylated by ribonucleotide reductase, forming its active state known as difluorodeoxycytidine triphosphate. This active form directly inhibits DNA synthesis and also hinders RNA synthesis. Gemcitabine′s mechanism of action primarily revolves around inhibiting ribonucleotide reductase, which is responsible for producing deoxyribonucleotides, the essential components of DNA.